The impact of different TiO2 particles on the activity of acetylcholinesterase

Jan, Zala (author), Jereb, Gregor (author), Kononenko, Veno (author), Drobne, Damjana (author)

Over the last decades, the production of nanoparticles (NPs) has been rapidly growing on the global scale. The data on biological activity of NPs is crucial in evaluating their safe use and in the development of new products containing NPs. Different tests are available for the purposes of determining interactions between NPs and biological systems. In this study, a modified Ellman’s method, adapted to work with microtiter plate, was used. This study aims to evaluate the biological reactivity of 10 different titanium dioxide (TiO2) particles with different primary sizes, crystallinity and different coatings. Biological reactivity was assessed by testing their adsorption to acetylcholinesterase (AChE) and to inhibit its activity. It was assumed that the tested particles inhibit AChE depending on physiochemical properties of particles, with crucial importance of their crystal structure. It was also assumed that a higher NP adsorption to AChE and a formation of so-called corona have no direct correlation to stronger AChE inhibition. TiO2 particles sized between 5 and 100 nm showed the highest rate of inhibition and adsorption to AChE, whereas the particles with size between 100 and 500 nm showed a lower rate of both. In our study the crystallinity of TiO2 particles did not have a significant effect on their ability to inhibit and adsorb AChE. Observed NP inhibition and adsorption to AChE was dependant on NP concentration. We observed higher NP effect on inhibition than on adsorption.

• Language: English
• Keywords: TiO2, acetylcholinesterase, inhibition, adsorption, nanoparticles
• Typology: 1.08 - Published Scientific Conference Contribution
• Organization: ZF - Faculty of Health Sciences
• Year: 2019
• Number of pages: Str. 64-70
• PID: 20.500.12556/RUL-113112
• UDC: 620.3:576.3
• COBISS.SI-ID: 5741419
• Publication date in RUL: 04.12.2019

Record is a part of a monograph

• Title: Sokratska predavanja : zbornik recenziranih prispevkov
• Editors: Veronika Kralj-Iglič
• Place of publishing: Ljubljana
• Publisher: Zdravstvena fakulteta, = Faculty of Health Sciences
• Year: 2019
• ISBN: 978-961-6808-94-1
• COBISS.SI-ID: 302959104

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 04.12.2019

Secondary language

• Language: Slovenian
• Keywords: TiO2, acetilholinesteraza, inhibicija, adsorpcija, nanodelci