High detection rate and high genetic diversity of genogroup I Picobirnaviruses from roe deer

Kuhar, Urška (author), Jamnikar Ciglenečki, Urška (author)

Picobirnaviruses (PBVs) have been characterized as opportunistic enteric pathogens detected in various domestic, zoo and wild animals, suggesting a wide host range of these viruses. It is thus important to monitor wild animals for the presence of various human and animal pathogens in order to identify a potential reservoir of infectious diseases. In this study, the first phylogenetic analysis of PBV from roe deer (Capreolus capreolus) was performed with a total of 70 investigated samples of feces from roe deer collected in 2014 and 2015 during a survey throughout Slovenia. A high detection rate of PBVs was observed with newly designed specific primers, 42 samples out of 70 (60%) being positive. Phylogenetic analysis of the partial RdRp gene showed that roe deer PBV sequences were distributed over the whole phylogenetic tree and were distributed between 7 highly supported groups and 12 separate branches within the PBV genogroup I. The animal PBV strain most closely related to roe deer PBV strains was the Rhesus macaque PBV/BGD/PbV-55 strain, with 89.1% nucleotide identity to that of PBV SLO/D80-14. Overall nucleotide sequence identity between PBV strains obtained from roe deer ranged from 60.4 to 100%, confirming the high genetic diversity with no subtypes related to host species or geographic location in general. This first phylogenetic survey of roe deer PBVs provides further knowledge concerning the putative host range and confirms the high genetic diversity of these PBVs.

• Language: English
• Keywords: Picobirnavirus, epidemiology, roe deer, phylogenetic analysis
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Author Accepted Manuscript
• Publisher: Elsevier
• Year: 2019
• Number of pages: Str. 210-213
• Numbering: Vol. 73
• PID: 20.500.12556/RUL-112701
• UDC: 639.111.1:57.083.2:575.86
• ISSN on article: 1567-1348
• DOI: 10.1016/j.meegid.2019.05.001
• COBISS.SI-ID: 4819322

Copyright

založnik

• Publication date in RUL: 06.11.2019

Record is a part of a journal

• Title: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
• Publisher: Elsevier
• ISSN: 1567-1348
• COBISS.SI-ID: 2319124

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
• Licensing start date: 06.11.2019

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana