izpis_h1_title_alt

Viscoelastic response of Escherichia coli biofilms to genetically altered expression of extracellular matrix components
ID Horvat, Maruša (Author), ID Pannuri, Archana (Author), ID Romeo, Tony (Author), ID Dogša, Iztok (Author), ID Stopar, David (Author)

.pdfPDF - Presentation file, Download (1000,49 KB)
MD5: 285874CC898F018A1287D20935E648C6
URLURL - Source URL, Visit https://pubs.rsc.org/en/content/articlelanding/2019/SM/C9SM00297A#!divAbstract This link opens in a new window

Abstract
How viscoelastic properties of the extracellular matrix affect the various biological functions conferred by biofilms is an important question in microbiology. In this study viscoelastic response of Escherichia coli biofilms to genetically altered expression of extracellular matrix components was studied. Biofilms of the wild type E. coli MG1655 and its mutant strains producing different amounts of extracellular matrix components (curli, colonic acid, poly-β-1,6-N-acetyl-D-glucosamine) were used to examine the viscoelastic behavior of biofilms grown at solid -atmosphere interface. The results suggest that the presence of curli proteins dominates biofilm mechanical behavior. The rheological data indicate that cohesive energy of the biofilm was highest in the wild type strain. The results demonstrate the importance of extracellular matrix composition for biofilm mechanical properties. We propose that by genetically altering the expression of extracellular matrix polymers bacteria are able to modulate the mechanical properties of their local environment in accordance with bulk environmental conditions.

Language:English
Keywords:biofilm, Escherichia coli, EPS, rheology, viscoelasticity
Work type:Scientific work (r2)
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:2019
Article version:Postprint, final article version, accepted into publication
Number of pages:Str. 5042-5051
Numbering:Vol. 15
PID:20.500.12556/RUL-111832 This link opens in a new window
UDC:579.24/.26:579.842:544
ISSN on article:1744-683X
DOI:10.1039/c9sm00297a This link opens in a new window
COBISS.SI-ID:5060728 This link opens in a new window
Publication date in RUL:15.10.2019
Views:699
Downloads:470
Metadata:XML RDF-CHPDL DC-XML DC-RDF
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Record is a part of a journal

Title:Soft matter
Publisher:Royal Society of Chemistry
ISSN:1744-683X
COBISS.SI-ID:19616551 This link opens in a new window

Secondary language

Language:Slovenian
Keywords:biofilmi, Escherichia coli, zunajcelični polimeri, reološke lastnosti, viskoelastičnost

Projects

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P4-0116
Name:Mikrobiologija in biotehnologija živil in okolja

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Back