Mikropropagacija gorenjskega nageljna (Dianthus sp. L.)

Doljak, Žiga (author), Luthar, Zlata (mentor)

V in vitro poskus smo vključili 6 rastlin gorenjskega nageljna (Dianthus sp.) iz katerih smo pripravili nodijske in listne izsečke ter jih razkužili z 2 % raztopino dikloroizocianurne kisline. Nodijske izsečke smo inokulirali na osnovno MS gojišče z dodatkom BAP in NAA (3 in 0,5 mg/l) - gojišče 1 ter enako koncentracijo BAP in NAA (1 mg/l) - gojišče 2. Listne izsečke smo inokulirali prav tako na osnovno MS gojišče z dodatkom BAP (2,5 mg/l) - gojišče 3 in gojišče 4 z BAP (5 mg/l). Na gojišču 1 je bila regeneracija 38,7 % in na gojišču 2 je bila za 6,3 % boljša (45 %). Na gojišču 1 smo zaradi okužbe izgubili 13,1 % izsečkov in poganjkov ter na gojišču 2 je bilo okuženih samo 7,7 % izsečkov in poganjkov. Nastale poganjke smo subkultivirali na gojišče za koreninjenje 5 z IBA (5 mg/l) in 24 % poganjkov se je uspešno koreninilo. Z namenom izboljšati fazo koreninjenja smo 135 poganjkov subkultivirali na gojišče za koreninjenje 6 z IAA (2,5 mg/l) in kar 45,8 % poganjkov je oblikovalo korenine. Koreninjeni poganjki so bili vitalni in primerni za aklimatizacijo. V prihodnje bi lahko preko meristemske in vitro kulture pridobivali brezvirusne sadike gorenjskega nageljna.

• Language: Slovenian
• Keywords: in vitro kultura, gorenjski nagelj, Diatnhus sp., genotip, nodijski izsečki, meristem, listni izsečki, gojišče, hormoni, regeneracija, koreninjenje
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [Ž. Doljak]
• Year: 2019
• PID: 20.500.12556/RUL-111352
• UDC: 582.661.1:57.082.2(043.2)
• COBISS.SI-ID: 9314425
• Publication date in RUL: 28.09.2019

Secondary language

• Language: English
• Title: Micropropagation of Gorenjski carnation (Dianthus sp. L.)

Abstract

We included 6 samples of Dianthus sp. in the in vitro experiment. From them we prepared leaf and node explants and disinfected them using a 2 % solution of dichloroisocyanuric acid. We inoculated node explants on a basic Murashige and Skoog’s (MS) growth medium enriched with BAP and NAA. The first medium was supplemented with 3 mg/l BAP and 0.5 mg/l NAA and the second included equimolar concentrations of both hormones (1 mg/l). Furthermore, we inoculated leaf explants on the same MS medium with added BAP in two different concentrations. 2.5 mg/l was added to medium 3 and 5 mg/l to medium 4. Growth medium 2 gave the highest regeneration response followed by medium 1 (45 % and 38.7 % respectively). There was a 13.1 % loss of shoot buds and explants due to infection on medium 1 and 7.7 % on medium 2. Newly formed adventitious shoot buds were subcultured on a root formation medium with IBA (5 mg/l). 24 % of shoot buds were successfully rooted in. We subcultured 135 shoots on a root formation medium with IAA (2.5 mg/l) with the purpose of improving root formation. As a result of this procedure 45.8 % shoot buds formed viable roots suitable for acclimatisation. Henceforth a possibility may arise to acquire virus – free saplings of Dianthus sp. through meristem in vitro cultures.

• Keywords: in vitro culture, carnation, Diatnhus sp., genotype, nodal explants, meristem, leaf explants, culture medium, hormones, regeneration, rooting