Molecular chirality and its pharmacological effects on human body have been important in pharmaceutical industry for quite a long time. Thus, pharmaceutical industry strives to produce pure enantiomeric drugs to mitigate or eliminate adverse side effects of unwanted enantiomers in the mix. These pure compounds can be obtained by two different approaches:
Separation of racemic mixture by chiral chromatography
Stereospecific synthesis
Topic of this master thesis was chiral separation with the help of high-performance liquid chromatography. A normal-phased system was used where we were changing parameters of chromatographic system with the goal of separation of gyrase inhibitors. Enantiomeric compo-unds were further used for analytical and preparative purposes.
We were trying to develop a method for chiral separation by changing composition of mobile phase on a chromatographic column Kromasil Chiral CelluCoat. Hexane, methanol and isopro-panol were used as a mobile phase. In a series of experiments, we successfully separated 4 compounds: enantiomeric couple 1 and 2, 7 and 8, 10 as well as 11. For the compounds 1 and 2 we have also determined enantiomeric excess. We have shown that no separation can be ma-de on described system with compounds: 3 and 4, 5 and 6 and also 9.
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