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Vpliv načinov rokovanja na stres pri laboratorijskih miših
Vengar, Ana (Author), Pirman, Tatjana (Mentor) More about this mentor... This link opens in a new window

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Abstract
Delo z laboratorijskimi živalmi zahteva dnevni stik miši s skrbnikom. Trenutno se za vsakodnevno rokovanje uporablja klasična metoda prijem za rep. Znano je, da prijem za rep mišim povzroča nelagodje in stres. Žival, ki je med izvajanjem poskusov pod stresom, ne bo izrazila naravnega obnašanja. To ima lahko vpliv na pridobljene rezultate. Uvajata se dve novi metodi rokovanja, ki naj bi bili za miši manj stresni ter spodbujali prostovoljno približevanje rokovalcu. To sta metoda rokovanja z odprto dlanjo in rokovanje s tunelom. Tekom diplomskega dela smo poskušali potrditi pozitiven vpliv obeh novih metod, tako da smo devet dni zaporedoma, izvajali vse tri prej omenjene načine rokovanja. Prvi, peti in deveti dan smo izvedli preizkus interakcije. Z merjenjem časa, ki so ga miši preživele v interakciji z rokovalcem smo določili vpliv posamezne metode. V poskusu smo uporabili tri različne linije ter oba spola živali. Tako smo lahko analizirali tudi vpliv linije in spola na prilagoditev posamezni metodi rokovanja. Izkazalo se je, da se miši najhitreje privadijo rokovanju s tunelom, sledi metoda z odprto dlanjo in kot zadnja je klasična metoda prijema za rep.

Language:Slovenian
Keywords:poskusne živali, laboratorijske miši, stres, dobrobit živali, rokovanje, metode rokovanja
Work type:Bachelor thesis/paper (mb11)
Organization:BF - Biotechnical Faculty
Year:2019
COBISS.SI-ID:4296840 Link is opened in a new window
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Downloads:15
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Secondary language

Language:English
Title:Effects of handling laboratory mice on stress
Abstract:
Working with laboratory animals requires daily contact of mice with the handler. At that moment the most common method to handle laboratory mice is to pick up and restrain the mouse by tail. Picking up by tail is known to cause the anxiety and stress. The animal, which is under pressure during the experiment, will not express its natural behavior. This can have an impact on the results. There are two new methods of handling that led to voluntary approach and low anxiety. These are handling by tunnel and cupping mice on the open hand. In the B. Sc. Thesis we tried to confirm positive influence of both new methods. We performed all handling methods of handling for nine consecutive days. We made the interaction test on the first, fifth and ninth day. We determine the influence of each method by measuring the time that mice stay in interaction with the handler. In the experiment, we used three different lines and both genders. So we were able to analyse the influence of the line and gender to the different handling methods. Results showed the best responses to the handling by tunnel, followed by open hand and as expected the last picking up by tail.

Keywords:laboratory animals, laboratory mice, stress, animal welfare, handling, handling methods

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