izpis_h1_title_alt

Sinteza novih fluorescentnih označevalcev biomolekul na osnovi z Cu0 katalizirane CuAIAC reakcije
ID Erjavec, Bibi (Author), ID Svete, Jurij (Mentor) More about this mentor... This link opens in a new window

.pdfPDF - Presentation file, Download (1,83 MB)
MD5: D2344EC3566F678005F3979723639ECF

Abstract
Na osnovi z bakrom katalizirane azometin imin-alkin cikloadicije smo sintetizirali pet novih, pod UV svetlobo fluorescentnih cilkloaduktov. Kot alkin smo uporabili inone sintetizirane iz anhidrida jantarne kisline ter iz propiolne kisline. Inoni so bili v namen vezave na aminske skupine biomolekul aktivirani ali z N-hidroksisukcinimidom (NHS) ali z benzotriazolom (BtH). Na inone smo ciklizirali različne N,N-ciklične azometin imine, reakcijo pa smo katalizirali z Cu0 v obliki bakrovega prahu ali bakrove žičke. Za začetni preizkus biokonjugacije smo z NHS in BtH aktivirane fluorescentne cikloadukte vezali na (aminometil)polistiren (AMP). Na AMP smo vezali tudi z NHS in BtH aktivirane ter jih v fluorescentne produkte z CuAIAC ciklizirali na (aminometil) polistirenu. Z BtH aktivirane cikloadukte smo vezali še na realno biomolekulo, goveji serumski albumin (BSA), ter merili emisijske spektre tako označenih proteinov.

Language:Slovenian
Keywords:CuAIAC, “klik” reakcija, biomarkerji, azometin imini
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Year:2019
PID:20.500.12556/RUL-110541 This link opens in a new window
COBISS.SI-ID:1538444995 This link opens in a new window
Publication date in RUL:16.09.2019
Views:1720
Downloads:189
Metadata:XML RDF-CHPDL DC-XML DC-RDF
:
Copy citation
Share:Bookmark and Share

Secondary language

Language:English
Title:Synthesis of novel fluorescent biomolecule markers based on Cu0 catalyzed CuAIAC reaction
Abstract:
We used copper-catalyzed azomethine imine–alkyne cycloaddition (CuAIAC) to prepare five novel cycloadducts that exhibited fluorescence under UV light. As alkynes in CuAIAC we used two ynones that we synthesized one from succinic anhydride and the other from propiolic acid. The ynones were activated with either N-hydroxysuccinimide (NHS) or benzotriazole (BtH) in order to facilitate a covalent bonding with amine groups of biomolecules, thus making bioconjugation possible. Ynones were cyclized with various N,N-cyclic azomethine imines and the reaction was catalyzed with Cu0 in the form of copper particles or copper wire. NHS and BtH activated fluorescent cycloadducts were first bonded to (aminomethyl)polystyrene (AMP) as a preliminary test of bioconjugation. We also bonded AMP with activated ynones and then performed CuAIAC on the ynone-marked AMP to again yield a fluorescent polystyrene. Lastly, we bonded BtH activated cycloadducts onto amine groups of bovine serum albumin (BSA) and measured their emission spectra.

Keywords:CuAIAC, »click« reaction, biomarkers, azomethine imines

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Back