Delovanje lektina kokaprina na patogene in probiotične bakterije

Kuret, Neja (author), Klančnik, Anja (mentor), Sabotič, Jerica (comentor)

Odpornost mikroorganizmov proti obstoječim protimikrobnim sredstvom postaja ena izmed glavnih težav javnega zdravstva. Protimikrobna sredstva so problematična tudi z vidika normalnega človeškega mikrobioma, saj zavrejo rast probiotičnih, za človeka koristnih bakterij. Lektini, proteini, ki se reverzibilno in specifično vežejo na ogljikove hidrate, imajo dokazano protibakterijsko aktivnost in potencialno predstavljajo alternativno strategijo nadzora ali preprečevanja okužb odpornih mikroorganizmov. V raziskovalnem delu smo pripravili lektin KKP iz gobe Coprinopsis cinerea s pomočjo bakterijskega ekspresijskega sistema ter preučevali njegov vpliv na rast in adhezijo patogenih in probiotičnih mikroorganizmov. Lektin KKP zmanjša kapaciteto rasti patogene bakterijske vrste Salmonella infantis, ob dodatku proteaznih zaviralcev pa zmanjša tudi adhezijo. Nasprotno, lektin KKP poveča adhezijo večine analiziranih probiotičnih bakterij, predvsem rodu Lactobacillus. Učinek lektina KKP je bil zelo podoben pri bakterijah istega rodu, različen pa pri bakterijah različnega rodu, kar nakazuje na zelo specifično delovanje. Lektin KKP se specifično veže na proteine celične stene oz. zunajcelične proteine bakterijah vrste Lactobacillus plantarum. Lektin KKP bi lahko uporabljali kot naravno alternativno protimikrobno snov za preprečevanje okužb z bakterijsko vrsto Salmonella infantis. Potencialno bi ga lahko uporabljali tudi kot naravno dopolnilo pri zdravljenju z obstoječimi protimikrobnimi sredstvi in na ta način ohranili rast in delovanje bakterij normalne mikrobiote.

• Language: Slovenian
• Keywords: biotehnologija, lektin kokaprin, patogene bakterije, probiotične bakterije, rast, adhezija
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [N. Kuret]
• Year: 2019
• PID: 20.500.12556/RUL-110441
• UDC: 606:61:579.6(043.2)
• COBISS.SI-ID: 9347193
• Publication date in RUL: 14.09.2019

KURET, Neja, 2019, Delovanje lektina kokaprina na patogene in probiotične bakterije [online]. Master’s thesis. N. Kuret. [Accessed 26 June 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=110441

Secondary language

• Language: English
• Title: The effect of lectin "kokaprin" on pathogenic and probiotic bacteria

Abstract

Antimicrobial resistance against commonly used antibiotics is one of the most serious global public health threats in this century. Moreover, the use of antibiotics also has detrimental effect on human intestinal microbiome diversity due to microbicidal activity against beneficial commensal bacteria. Lectins are proteins that specifically and reversibly bind to carbohydrates and exert anti-microbial activity, potentially representing an alternative strategy to fight against infections by resistant microbial strains. In our study, we recombinantly expressed and isolated lectin KKP from Coprinopsis cinerea. We evaluated its effect on growth and adhesion of pathogenic and commensal microorganisms. Lectin KKP reduced the growth capacity of pathogenic bacteria Salmonella infantis and an additive effect of KKP and protease inhibitors was observed reducing the adhesion of this bacteria. In contrast, lectin KKP augmented the adhesion of commensal bacteria, especially Lactobacillus species. The overall effect of lectin KKP on growth and adhesion of microorganisms was very similar for the strains of the same species but varied significanly between different species, pointing towards very specific target recognition and binding. We proved that lectin KKP specifically binds to the cell wall proteins and extracellular proteins of Lb. plantarum strains. In summary, lectin KKP could potentially be used as a natural, alternative anti-microbial substance against Salmonella infantis infection. Furthermore, it could also be used as a natural supplement to antibiotic therapy that preserves the growth and fuction of commensal bacteria.

• Keywords: lectin kokaprin, probiotic bacteria, pathogenic bacteria, growth, adhesion