izpis_h1_title_alt

Odgovor mišjih tumorskih modelov na terapijo z večbarvnim imunofluorescenčnim barvanjem
Adamič, Urša (Author), Čemažar, Maja (Mentor) More about this mentor... This link opens in a new window, Markelc, Boštjan (Co-mentor)

.pdfPDF - Presentation file, Download (12,61 MB)

Abstract
Za zdravljenje raka je na voljo veliko različnih terapij. Tumor je zgrajen iz tumorskih celic in t.i. tumorskega mikrookolja, ki ga sestavljajo raznolike celice in pomembno vpliva na razvoj tumorja. Tumorsko mikrookolje prispeva k sposobnosti metastaziranja in sodeluje pri učinkovitosti imunskega sistema, da tumor prepozna ali ga skrije. Z metodo večbarvnega imunofluorescenčnega barvanja specifično označimo več različnih tipov celic na isti rezini. V raziskovalnem delu naloge smo označili žile, mesta proliferacije, makrofage, citotoksične limfocite T in celice pomagalke T. Barvali smo dva različna mišja tumorska modela, in sicer celice mišjega melanoma (B16F10) in celice mišjega raka debelega črevesa in danke (CT26). Največ makrofagov je bilo okrog in v tumorju po terapiji z genskim elektroprenosom interlevkina-12. Žile so se dobro barvale v vseh tumorjih, medtem ko smo limfocite T v sledeh opazili le pri tumorskem modelu CT26. Barvanje je bolje potekalo na zmrznjenih rezinah tumorja CT26. Velikosti vseh tumorjev so se po terapijah zmanjšale, oziroma so se na mestih tumorja pojavile nekroze. Na osnovi rezultatov lahko zaključimo, da so za večbarvno imunohistološko barvanje primernejše zmrznjene rezine tkiva. Pri obeh tumorskih modelih, po vseh načinih terapij, katerih del je bil tudi genski elektroprenos interlevkina-12, smo dokazali povečano količino makrofagov okoli tumorjev in v samih tumorjih.

Language:Slovenian
Keywords:biologija, rak, tumorsko mikrookolje, miši, IHC, onkologija
Work type:Master's thesis/paper (mb22)
Organization:BF - Biotechnical Faculty
Year:2019
Views:45
Downloads:23
Metadata:XML RDF-CHPDL DC-XML DC-RDF
 
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Secondary language

Language:English
Title:Response of mouse tumor models to therapy using multispectral immunofluorescent imaging
Abstract:
There are many different therapies available for treating cancer. Inside the body, tumor is composed of tumor cells that are surrounded by the tumor microenvironment, which is made up of diverse cells and has a significant effect on tumor development. The tumor microenvironment contributes to the metastasis ability and participates in the immune system's ability to recognize or hide the tumor. Using the multispectral immunofluorescence staining method, we label several different cell types on the same tissue slice. In the experimental part of the thesis, we labeled veins, proliferation sites, macrophages, cytotoxic T lymphocytes, and helper T cells. We stained two different mouse tumor models, namely, mouse melanoma cells (B16F10) and colon and rectal cancer cells (CT26). Most macrophages were located around the tumor after interleukin-12 gene transfer therapy. The veins stained well in all tumors, while the only traces of T lymphocytes were observed in the CT26 tumor. Staining was better performed on CT26 tumor slices stored at -20 ° C and thawed. The sizes of all tumors decreased or necrosis occurred after the therapies. Based on the results, we can conclude that frozen tissue slices are more suitable for multispectral immunofluorescent imaging. In both tumor models, increased amounts of macrophages have been demonstrated around and inside the tumors after all therapies involving interleukin-12 gene transfer.

Keywords:biology, cancer, tumor microenvironment, mice, IHC, oncology

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back