Knowing the exact content of biogenic amines in samples is relevant in different fields. Recent studies in the field of human health have shown that certain polyamines have antioxidative properties, positively affect cellular functions and slow down aging. Some types of food already contain certain biogenic amines in low concentrations, which increase when the food is no longer suitable for consumption. Therefore, the development of a selective, reliable and robust analytical method with a low limit of detection for determination of biogenic amines is of great importance. In the master's thesis, the influence of different parameters on the derivatisation with dansyl chloride was investigated. The derivatization allows the biogenic amines to be separated with liquid reverse-phase chromatography and to be detected by UV/Vis and fluorescence detectors. A method for determining the biogenic amines agmatine, tryptamine, β-phenethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine in the concentration range 0,1 – 1,0 mg/L was optimised. In addition, oxidative degradation of spermine and spermidine was studied with H2O2 and KMnO4. On the basis of mass spectra, it was proven that spermine degrades to putrescine, while spermidine degrades to putrescine and 1,3-diaminopropane.
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