Vpliv gojišča na rast glive Cordyceps militaris in njeni biološki učinki na kvasovko Saccharomyces cerevisiae

Turnšek, Primož (author), Jamnik, Polona (mentor), Pohleven, Franc (comentor)

Gliva Cordyceps militaris ima v tradicionalni kitajski medicini dolgo zgodovino uporabe, dandanes pa postaja pomembna kot prehransko dopolnilo tudi na zahodu. Mnoge sodobne raziskave so potrdile terapevtske učinke te glive. Raziskave kažejo, da imajo biološko aktivne snovi glive C. militaris, kot je kordicepin, mnoge pozitivne farmakološke učinke na zdravje ljudi. V naši raziskavi smo preučevali vpliv vrste gojišča na rast glive, proizvodnjo kordicepina in biološki učinek vodnih ekstraktov preraščenega gojišča. Micelij glive smo gojili na pšeničnem gojišču z dodanim peptonom, ličinkami mokarja ali kavnim ostankom. Vrsta gojišča je močno vplivala na rast glive C. militaris. Micelij je na pšeničnem gojišču z dodanimi mokarjevimi ličinkami rastel najbolj zgoščeno, medtem ko je na pšeničnem gojišču z dodanim kavnim ostankom tvoril največ primordijev. Iz vzorcev preraščenega gojišča, ki smo jih vzorčili v treh časovnih točkah, smo pripravili vodni ekstrakt in izmerili koncentracijo kordicepina. Ugotovili smo, da je na vsebnost kordicepina imela največji vpliv sestava gojišča, pri čemer sta se najboljše izkazali pšenično gojišče z dodanimi mokarjevimi ličinkami in pšenično gojišče z dodanim kavnim ostankom. Vodni ekstrakt preraščenega pšeničnega gojišča z dodanim kavnim ostankom je glede na pšenično gojišče brez dodatkov v celicah kvasovke Saccharomyces cerevisiae imel največji antioksidativni učinek in najmanj zmanjšal znotrajcelično raven ATP glede na kontrolo.

• Language: Slovenian
• Keywords: glive, Cordyceps militaris, bioaktivne spojine, rast, gojišča, kordicepin, znotrajcelična raven ATP, znotrajcelična oksidacija, Saccharomyces cerevisiae
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [P. Turnšek]
• Year: 2019
• PID: 20.500.12556/RUL-109728
• UDC: 582.282:615.334:577.1/.2
• COBISS.SI-ID: 5094264
• Publication date in RUL: 07.09.2019

TURNŠEK, Primož, 2019, Vpliv gojišča na rast glive Cordyceps militaris in njeni biološki učinki na kvasovko Saccharomyces cerevisiae [online]. Master’s thesis. P. Turnšek. [Accessed 18 May 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=109728

Secondary language

• Language: English
• Title: Influence of substrate on growth of fungus Cordyceps militaris and its biological effects on the yeast Saccharomyces cerevisiae

Abstract

In traditional Chinese medicine the Cordyceps militaris has a long history of use and is gaining importance as a food supplement in the west. Many modern studies confirm its therapeutic effects. Evidence shows that bioactive ingredients of the C. militaris have many positive pharmacological effects on human health. In our study, we investigated the influence of substrate type on growth of fungus, production of cordycepin and biological effect of water extracts from overgrown substrate. The mycelium was grown on wheat grains only and with addition of peptone, mealworm larvae or coffee waste. Type of substrate had a strong infrulence on growth of C. militaris. Mycelium growth was most condensed on wheat grains with added mealworm larvae. Primordia formation was strongest on wheaten grains with added coffee waste. Samples of the overgrown substrate were taken at three different growth periods. Water extracts were made from samples and concentration of cordycepin was measured. We showed that type of substrate had the most important effect on concentration of cordycepin. Substrates from wheaten grains with added mealworm larvae and added coffee waste had resulted in biggest production of cordycepin. Water extract of overgrown wheat grains with added coffee waste had maximum effect on reduction of intracellular oxidation and minimum effect on reduction of intracellular ATP level in yeast cells of Saccharomyces cerevisiae, compared to wheat grains substrate.

• Keywords: fungi, Cordyceps militaris, bioactive compounds, growth, substrate, cordycepin, intracellular ATP level, intracellular oxidation, Saccharomyces cerevisiae