Termodinamske osnove dvofaznih vodnih sistemov

Pirc, Matija (author), Podgornik, Aleš (mentor)

Princip dvofaznega vodnega sistema se uporablja za frakcionacijo tekoče-tekoče, ki se je zaradi uporabe pri ekstrakciji, ločevanju, čiščenju in obogatitvi beljakovin, membran, virusov in drugih biomolekul razširila tako na laboratorijskem nivoju kot tudi v industriji. Ključne lastnosti tega načina separacije so prijazno okolje za biološke makromolekule, visok izkoristek in možnost enostavnega prenosa procesa na proizvodni nivo (»scale up«), prav tako pa je okolju prijazna ter finančno ugodna metoda . Opisali bomo njene temeljne lastnosti, postopek separacije faz in termodinamske osnove dvofaznih vodnih sistemov.

• Language: Slovenian
• Keywords: termodinamika, ekstrakcija biomolekul, separacija faz
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2019
• PID: 20.500.12556/RUL-109680
• COBISS.SI-ID: 1538326211
• Publication date in RUL: 06.09.2019

Secondary language

• Language: English
• Title: Thermodynamic basis of aqueous two-phase systems

Abstract

The principle of the aqueous two-phase system is used for liquid-liquid fractionation, which has expanded due to its use in extraction, separation, purification and enrichment of proteins, membranes, viruses and other biomolecules, both in laboratory and on industrial level. This separation method provides a friendly environment for macromolecules, high efficiency and the possibility of scaling up the process to an industrial level. It is environmentally friendly and a financially favourable method. We will focus in describing the basic properties of aqueous two-phase systems, the process of phase partitioning and thermodynamic basis of this method.

• Keywords: thermodynamics, biomolecule extraction, phase partitioning