IONSKA KROMATOGRAFIJA ZA DOLOČEVANJE ANIONOV

Tomšič, Rok (author), Kolar, Mitja (mentor)

V okviru diplomskega dela na Fakulteti za kemijo in kemijsko tehnologijo, Univerze v Ljubljani, je bil vpeljan sistem za anionsko izmenjevalno kromatografijo s konduktometrično detekcijo. S sistemom so bile izvedene analize realnih vzorcev v katerih smo določali: fluoridne, kloridne, nitritne, bromidne, nitratne, fosfatne in sulfatne ione. Metoda ionske kromatografije je bila tudi delno validirana, saj smo študirali linearnost ter določili LOD in LOQ. Določali smo primernost oz. vpliv kromatografskih pogojev kot so: pretok in sestava, pH ter starost eluenta. Eksperimentalno delo je vključevalo tudi regeneracijo kolone, s katero smo želeli doseči manjšo asimetričnost vrhov, ustrezno ločitev vrhov, ki pripadata fosfatnim in sulfatnim ionom ter odpraviti zamike retencijskih časov.

• Language: Slovenian
• Keywords: ionska kromatografija, anioni, validacija, regeneracija kolone
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2019
• PID: 20.500.12556/RUL-109665
• COBISS.SI-ID: 1538327747
• Publication date in RUL: 06.09.2019

TOMŠIČ, Rok, 2019, IONSKA KROMATOGRAFIJA ZA DOLOČEVANJE ANIONOV [online]. Bachelor’s thesis. [Accessed 14 June 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=109665

Secondary language

• Language: English

Abstract

As a part of the diploma thesis at the Faculty of Chemistry and Chemical Technology of the University of Ljubljana, an anion exchange chromatography system with conductometric detection was introduced. The system performed analyses of real samples in which we determined: fluoride, chloride, nitrite, bromide, nitrate, phosphate and sulphate ions. The ion chromatography method was also partially validated, as we studied linearity and determined LOD and LOQ. We determined the suitability or the influence of the chromatographic conditions such as: flow and composition, pH and age of the eluent. The experimental work also included the regeneration of the column, which was aimed at reducing the peak asymmetry of the peaks, the proper separation of the peaks belonging to the phosphate and sulphate ions, and to eliminate the retention time delays.

• Keywords: ion chromatography, anions, validation, column regeneration