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Preučevanje biofilma bakterije Enterococcus faecalis Symbioflor 1 na modelnih zobnih površinah
Hudej, Lara (Author), Stopar, David (Mentor) More about this mentor... This link opens in a new window, Dogša, Iztok (Co-mentor)

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Abstract
Ob vnetju ali okužbi zobne pulpe je potrebno koreninsko zdravljenje. Vzrok za okužbo ali vnetje so lahko zobni karies, poškodbe zobnih tkiv ali pa sam stomatološki poseg. Mikroorganizem, ki je povezan z neuspelimi endodontskimi zdravljenji, je Enterococcus faecalis, ki velja za eno izmed zelo odpornih bakterij proti protimikrobnim sredstvom in drugim načinom odstranjevanja bakterij. Ima širok razpon fizikalno-kemijskih dejavnikov, pri katerih raste in lahko preživi ekstremne pogoje. V magistrski nalogi smo preverjali sev E. faecalis Symbioflor 1, ki ima probiotično delovanje. Določili smo optimalne fizikalno-kemijske pogoje za rast seva v planktonski obliki in spremljali rast biofilmov na različnih površinah (navadno objektno stekelce, pleksi steklo, model zobnega kanala, dentin). Dodatno smo preverili, kako vpliva na odstranjevanje biofilmov 5 % raztopina natrijevega hipoklorita. Rezultati so pokazali nastanek in dobro rast biofilmov na vseh testnih površinah. Ugotovili smo, da spiranje z natrijevim hipokloritom odstrani večino celic in spremeni mehanske lastnostni površine dentina. Spiranje je bilo manj učinkovito na površinah, kjer so bile prisotne površinske poškodbe.

Language:Slovenian
Keywords:biofilmi, Enterococcus faecalis Symbioflor 1, planktonska oblika, natrijev hipoklorit, odstranjevanje biofilmov, zobna pulpa
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2019
Publisher:[L. Hudej]
UDC:579.24+579.26:616.314.1
COBISS.SI-ID:5089400 Link is opened in a new window
Views:376
Downloads:136
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Secondary language

Language:English
Title:A study of the bacterium Enterococcus faecalis Symbioflor 1 biofilm on model dental surfaces
Abstract:
In case of inflammation or infection of the tooth pulp, root treatment is required. The cause of infections or inflammations may be dental caries, dental tissue damage, or a dental intervention itself. The microorganism associated with unsuccessful endodontic treatments is Enterococcus faecalis, which is considered one of the most resistant bacteria against antimicrobial agents and other methods of bacteria removal. It can survive extreme conditions due to its significant growth range which also contributes to high resistance of the bacterium. This thesis tests the probiotic strain Enterococcus faecalis Symbioflor 1, which also shows significant growth range and antimicrobial resistance. Optimal physico-chemical conditions for the growth of the strain in the planktonic form were tested and the biofilm growth on various surfaces (regular glass slide, plexiglass slide, model of a tooth canal, dentine) was monitored. The effectiveness of the 5 % sodium hypochlorite solution in removing the biofilm was also examined. The results demonstrated successful formation and growth of the biofilms on all test surfaces. It was discovered that washing with the sodium hypochlorite solution removes most of the cells and even alters the mechanical properties of the dentine. Washing was equally effective on other surfaces, except on the regular slide where defects were present.

Keywords:biofilms, Enterococcus faecalis Symbioflor 1, planctonic culture, sodium hypochlorite, irradiation systems on biofilms, tooth pulp

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