Določanje kofeina v prehranskih dopolnilih

Šenica, Leja (author), Zupančič, Tatjana (mentor)

Na trgu je na voljo veliko število prehranskih dopolnil in napitkov, ki vsebujejo kofein. Kofein je naravni alkaloid, ki ga najdemo v listih, plodovih in semenih mnogih rastlin. Ljudje ga radi uživajo zaradi njegovega poživljajočega učinka, ki zmanjšuje utrujenost in zaspanost. V diplomskem delu sem določala vsebnost kofeina v prehranskih dopolnilih in različnih pijačah oziroma kavah in čaju. Za določanje kofeina sem uporabila reverzno-fazno tekočinsko kromatografijo visoke ločljivosti z UV detektorjem. Tekom eksperimentalnega dela sem preverila tudi ponovljivost kromatografske metode. Prišla sem do zaključka, da največ kofeina vsebuje prehransko dopolnilo Guarana natural energy, najmanj pa zeleni čaj.

• Language: Slovenian
• Keywords: HPLC, kofein, prehranska dopolnila, topli napitki
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2019
• PID: 20.500.12556/RUL-109507
• COBISS.SI-ID: 1538312387
• Publication date in RUL: 04.09.2019

ŠENICA, Leja, 2019, Določanje kofeina v prehranskih dopolnilih [online]. Bachelor’s thesis. [Accessed 25 March 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=109507

Secondary language

• Language: English
• Title: Determination of caffeine in dietary supplements

Abstract

A large number of dietary supplements and beverages containing caffeine are available on the market. Caffeine is a natural alkaloid found in leaves, fruits and seeds of many plants. People like to drink caffeine because of its stimulating effect, which reduces exhaustion and sleepiness. In this diploma thesis I aimed to determine the caffeine content in various dietary supplements and drinks (coffee and tea). A reversed-phase high performance liquid chromatography with UV detector was used for determination of caffeine. I also checked repeatability of the chromatographic method. I came to conclusion that Guarana natural energy supplement contains the most caffeine, while green tea turned out to be the least caffeinated.

• Keywords: HPLC, caffeine, dietary supplements, hot beverages