Etiološka vloga človeških papilomavirusov pri nastanku papilomov ustne votline

Janc, Mojca (author), Poljak, Mario (mentor), Hošnjak, Lea (comentor)

Okužba z različnimi genotipi človeških papilomavirusov (HPV) je povezana z nastankom benignih novotvorb ploščatoceličnega epitelija na številnih delih telesa, tako v anogenitalnem predelu, na koži, na očesni veznici kot tudi v grlu. Okužba s HPV se kot eden izmed možnih etioloških dejavnikov omenja tudi v povezavi z nastankom raznolikih benignih novotvorb ustne votline, med katerimi so najbolj pogosti ploščatocelični papilomi. V do sedaj objavljenih raziskavah se prevalenca okužbe s HPV v omenjenih novotvorbah močno razlikuje. V sklopu magistrske naloge smo z uporabo molekularnih metod za dokazovanje in genotipizacijo DNA HPV želeli določiti prevalenco okužbe s HPV v 343 arhivskih tkivnih vzorcih papilomov ustne votline. Za opredelitev etiološke povezave med virusno okužbo in nastankom sprememb smo rezultate metod za dokazovanje in genotipizacijo HPV dopolnili z rezultati hibridizacije in situ (ISH) in oceno virusnega bremena. Z uporabo GP5+/6+/68 verižne reakcije s polimerazo (PCR) ter Gamma-HPV PCR v kombinaciji s sekveniranjem, HPV-204 PCR v realnem času (RT-PCR) in HPV-1/-63 RT-PCR, smo DNA HPV dokazali v 58 od 343 (16,9 %) vzorcev papilomov ustne votline, pri čemer smo v 34/58 (58,6 %) primerov dokazali prisotnost genotipa HPV-6, dodatno pa smo v posameznih primerih dokazali še HPV-1, -2, -10, -11, -13, -16, -27, -32, -44 (podtip HPV-55) ter -74. Iz kombinacije rezultatov dokazovanja prisotnosti DNA HPV, ISH in ocene virusnega bremena lahko sklepamo, da je HPV-6 najverjetnje povezan z nastankom 10 % ploščatoceličnih papilomov ustne votline. Manj kot 1 % ploščatoceličnih papilomov ustne votline pa je najverjetneje povezan z okužbo s HPV-11 oziroma HPV-44 (podtip HPV-55).

• Language: Slovenian
• Keywords: človeški papilomavirusi, benigne novotvorbe, papilomi, ustna votlina, molekularne tehnike, PCR
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [M. Janc]
• Year: 2019
• PID: 20.500.12556/RUL-109262
• UDC: 578.7:616-006.52:577.2.083
• COBISS.SI-ID: 5088120
• Publication date in RUL: 29.08.2019

Secondary language

• Language: English
• Title: Etiological role of human papillomaviruses in the development of oral papillomas

Abstract

Human papillomaviruses (HPV) are commonly found in benign neoplasms of a wide range of epithelial surfaces. Different HPV genotypes are associated with benign lesions, including common warts, anogenital warts, conjunctival squamous cell papillomas and laryngeal papillomas. HPVs are presented as putative etiological agents of various benign oral squamous cell lesions, including squamous cell papillomas, with HPV prevalence ranging from 0 to 100% in previous studies. The aim of the present study was to investigate the potential etiological role of HPV in oral papillomas, by determining the prevalence of HPV infections in a collection of 343 archival tissue specimens. The presence of HPV infection was determined in 58/343 (16.9%) tissue samples, using GP5+/6+/68 polymerase chain reaction (PCR) and Gamma-HPV PCR in combination with Sanger sequencing, HPV-204 real-time PCR (RT-PCR) and HPV-1/-63 RT-PCR. In our collection of samples, the most frequently detected HPV was HPV-6 (34/58; 58.6%), followed by HPV-1, -2, -10, -11, -13, -16, -27, -32, -44 (Subtype HPV-55) and -74 in sporadic cases of oral squamous cell papillomas. The combination of different HPV detection methods used, in situ hybridization results and viral load estimations, obtained in the present study, suggest that HPV-6 could be etiologically associated with the development of approximately 10% of oral squamous cell papillomas. Furthermore, the results suggest that oral squamous cell papillomas can be caused by HPV-11 or HPV-44 (Subtype HPV-55) infection in less than 1% of cases.

• Keywords: human papillomavirus, benign neoplasms, papillomas, oral cavity, molecular techniques, PCR