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Salmonella Infantis in broiler flocks in Slovenia : the prevalence of multidrug resistant strains with high genetic homogeneity and low biofilm-forming ability
Pate, Mateja (Author), Mićunović, Jasna (Author), Golob, Majda (Author), Vestby, Lene Karine (Author), Ocepek, Matjaž (Author)

URLURL - Source URL, Visit https://www.hindawi.com/journals/bmri/2019/4981463/ This link opens in a new window

Abstract
For almost a decade, the number of Salmonella enterica subspecies enterica serovar Infantis-positive broiler flocks has been steadily increasing in Slovenia, doubling the number of positive holdings in only a few years. Since multidrug resistant S. Infantis isolates are highly prevalent in the broiler meat industry and may represent a public health concern through the food chain, we aimed to investigate the antimicrobial susceptibility, genetic diversity, and biofilm-forming ability of S. Infantis from Slovenian broiler flocks. A total of 87 S. Infantis strains isolated from broiler faeces in the period between 2007 and 2013 were studied. The samples originated from 41 farms which were subcontractors of three major food business operators and from two autonomously operating holdings (farms). Isolates were phenotypically tested for their susceptibility to 14 antimicrobials from nine classes by determining the minimum inhibitory concentration with the microdilution method. Only 8% of the isolates were susceptible to all of the antimicrobial agents tested, while 88.5% of the isolates were multidrug resistant, with the most common resistance pattern CipNxSSuT (65.5%) followed by CipNxSuT (17.2%). Pulsed-field gel electrophoresis (PFGE) divided the strains into five clusters (A-E) comprising 16 distinct XbaI PFGE profiles. Sixty-five out of 87 isolates were grouped in clusters A and B, with the predominant PFGE profiles A1 and B1 encompassing 33 and 28 isolates, respectively. A vast majority of the isolates (75/87) showed >90% PFGE profile similarity. The biofilm-forming capacity of the tested isolates, determined with crystal violet assay in polystyrene microwell plates, was generally weak. The average biofilm formation for persistent strains was higher than for presumably nonpersistent strains; however, the difference was not significant. It seems that S. Infantis persistence on broiler farms is more related to its widespread occurrence in the broiler production chain and ineffective disinfection protocols than to its ability to form biofilm.

Language:English
Keywords:Poultry diseases-drug therapy, Salmonella infections, animal-microbiology, biofilms-drug effects, chickens-microbiology, drug resistance, multiple, bacterial, feces-microbiology, genetic variation, microbial sensitivity tests, serogroup, Slovenia
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Year:2019
Publisher:Hindawi
Number of pages:Art. ID 4981463, str. 1-13
Numbering:Vol. 2019
UDC:636.09:579.62:614
ISSN on article:2314-6141
DOI:10.1155/2019/4981463 Link is opened in a new window
COBISS.SI-ID:4756602 Link is opened in a new window
License:CC BY 4.0
This work is available under this license: Creative Commons Attribution 4.0 International
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Downloads:106
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Record is a part of a journal

Title:BioMed research international
Shortened title:Biomed Res Int
Publisher:Hindawi Pub. Co.
ISSN:2314-6141
COBISS.SI-ID:30624729 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P4-0092
Name:Zdravje živali, okolje in varna hrana

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