Salmonella Infantis in broiler flocks in Slovenia : the prevalence of multidrug resistant strains with high genetic homogeneity and low biofilm-forming ability

Pate, Mateja (author), Mićunović, Jasna (author), Golob, Majda (author), Vestby, Lene Karine (author), Ocepek, Matjaž (author)

For almost a decade, the number of Salmonella enterica subspecies enterica serovar Infantis-positive broiler flocks has been steadily increasing in Slovenia, doubling the number of positive holdings in only a few years. Since multidrug resistant S. Infantis isolates are highly prevalent in the broiler meat industry and may represent a public health concern through the food chain, we aimed to investigate the antimicrobial susceptibility, genetic diversity, and biofilm-forming ability of S. Infantis from Slovenian broiler flocks. A total of 87 S. Infantis strains isolated from broiler faeces in the period between 2007 and 2013 were studied. The samples originated from 41 farms which were subcontractors of three major food business operators and from two autonomously operating holdings (farms). Isolates were phenotypically tested for their susceptibility to 14 antimicrobials from nine classes by determining the minimum inhibitory concentration with the microdilution method. Only 8% of the isolates were susceptible to all of the antimicrobial agents tested, while 88.5% of the isolates were multidrug resistant, with the most common resistance pattern CipNxSSuT (65.5%) followed by CipNxSuT (17.2%). Pulsed-field gel electrophoresis (PFGE) divided the strains into five clusters (A-E) comprising 16 distinct XbaI PFGE profiles. Sixty-five out of 87 isolates were grouped in clusters A and B, with the predominant PFGE profiles A1 and B1 encompassing 33 and 28 isolates, respectively. A vast majority of the isolates (75/87) showed >90% PFGE profile similarity. The biofilm-forming capacity of the tested isolates, determined with crystal violet assay in polystyrene microwell plates, was generally weak. The average biofilm formation for persistent strains was higher than for presumably nonpersistent strains; however, the difference was not significant. It seems that S. Infantis persistence on broiler farms is more related to its widespread occurrence in the broiler production chain and ineffective disinfection protocols than to its ability to form biofilm.

• Language: English
• Keywords: Poultry diseases-drug therapy, Salmonella infections, animal-microbiology, biofilms-drug effects, chickens-microbiology, drug resistance, multiple, bacterial, feces-microbiology, genetic variation, microbial sensitivity tests, serogroup, Slovenia
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Publisher: Hindawi
• Year: 2019
• Number of pages: Art. ID 4981463, str. 1-13
• Numbering: Vol. 2019
• PID: 20.500.12556/RUL-108777
• UDC: 636.09:579.62:614
• ISSN on article: 2314-6141
• DOI: 10.1155/2019/4981463
• COBISS.SI-ID: 4756602
• Publication date in RUL: 23.07.2019

Record is a part of a journal

• Title: BioMed research international
• Shortened title: Biomed Res Int
• Publisher: Hindawi Publishing
• ISSN: 2314-6141
• COBISS.SI-ID: 30624729

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 23.07.2019

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana