A group of vitamin B is classified as water-soluble vitamins that are not stored in the body and must be obtained with food on a daily basis.
The purpose of the master's thesis was to develop an analytical procedure based on HPLC-UV, which enables detection of nine B complex vitamins in dietary supplements at the same time. We performed the validation of the analytical procedure by validating the following validation parameters: linearity, robustness, selectivity, limit of detection, limit of quantification and stability.
The validated analytical procedure was used on four dietary supplements to test the content and uniformity of the vitamin B complex content. We also carried out a European Pharmacopoeia release test with an apparatus 1 with baskets. The purpose of the release test was to determine whether the supplements had a real prolonged release, as can be deduced from the data of their wrappings.
The analytical procedure we have developed allows us to do this within 30 minutes. We confirmed that our analytical procedure is suitable for the evaluation of B complex vitamins in samples. The results of the content test in the vast majority correspond to the prescribed criteria. We detected all the vitamins that have the declared value in all the samples. In the release test, no biotin was detected in any product due to its low concentrations. The results showed that the tested tablets do not have retard release according to the prescribed USP criterion.
The whole experimental part has been conducted in accordance with the good manufacturing practice.