Primerjava sestave komercialno dostopnih eteričnih olj čajne mirte (Melaleuca alternifolia) s tankoplastno, kolonsko in plinsko kromatografijo

Jakaj, Sara (author), Obreza, Aleš (mentor), Janeš, Damjan (co-mentor)

Eterično olje čajne mirte (Melaleuca aetheroleum) uporabljamo v aromaterapiji in parfumeriji zaradi značilnega ostrega, a hkrati prijetnega vonja. Njegovo protimikrobno in posledično antiseptično delovanja izkoriščamo tudi v medicinske namene. V okviru diplomske naloge smo med seboj primerjali sestavo eteričnih olj dveh proizvajalcev, avstralskega in nemškega. Za primerjavo sestave smo uporabljali tankoplastno kromatografijo. S kolonsko kromatografijo smo izolirali nekatere sestavine obeh eteričnih olj, ki smo jih kasneje identificirali s plinsko kromatografijo, sklopljeno z masnim spektrometrom. Ugotovili smo, da obe eterični olji ustrezata predpisom v Evropski farmakopeji, vendar vsebuje eterično olje nemškega proizvajalca manj sestavin, zlasti bolj polarnih. Rezultati so pokazali, da eterični olji vsebujeta največ monoterpenov in monoterpenoidov, vključno z 1,8-cineolom, terpinen-4-olom in ?-terpineolom, ki smo jih med eksperimentalnim delom uporabljali kot referenčne spojine. Na osnovi dobljenih rezultatov smo iz komercialno dostopnih sestavin izdelali zmes, ki naj bi bila po vonju čim bolj podobna eteričnemu olju čajne mirte. Uspešnost umetno pripravljenega EO, smo potrdili s pomočjo vonjalne analize, kjer so anketiranci potrdili podobnost vonjav z originaloma. Izdelali smo tudi hladilni hidrofilni gel z eteričnim oljem čajne mirte, ki je ohranil tekočo konsistenco.

• Language: Slovenian
• Keywords: eterično olje, čajna mirta, tankoplastna kromatografija, kolonska kromatografija
• Work type: Bachelor thesis/paper
• Organization: FFA - Faculty of Pharmacy
• Year: 2019
• PID: 20.500.12556/RUL-108558
• Publication date in RUL: 06.07.2019

Secondary language

• Language: English
• Title: Comparison of commercially available tea tree (Melaleuca alternifolia) essential oils composition by thin-layer, column and gas chromatography

Abstract

Tea tree essential oil of Melaleuca alternifolia is used in aromatherapy and perfumery. Due to its typical pleasant aroma and its antibacterial and antiseptic activity it is also used for medical purpose. This diploma thesis includes the comparison of composition of two essential oils, of Australian and German producer. We used thin-layer chromatography for the comparison of contents and column chromatography for isolation of various components of essential oils, which were later identified by gas chromatography, coupled with a mass spectrometer. We found out that both essential oils correspond to regulations of the European Pharmacopoeia, however the essential oil of the German producer has less ingredients, especially those that are more polar. Both essential oils mostly contain monoterpenes and monoterpenoids, including 1,8-cineol, terpinen-4-ol and alpha-terpineol, which were used as reference compounds during the experimental work. Based on the obtained results, we prepared a mixture of commercially available ingredients, which was supposed to be similar to Tea Tree Essential Oil. The effectiveness of artificially synthesized essential oil was confirmed by fragrance analysis. We also made a hydrophilic gel with tea tree essential oil, which retained its liquid consistency.

• Keywords: essential oil, tea tree Melaleuca alternifolia, thin-layer chromatography, column cromatography