Vpliv estradiola in prolaktina na izražanje sinaptotagmina IV v hipofizi podgan

Petrič, Patricia (author), Živin, Marko (mentor), Zorović, Maja (co-mentor)

Hipofiza je žleza z notranjim izločanjem, ki nadzira in uravnava delovanje drugih žlez. V njej se nahajajo laktotrofi, celice, ki izločajo hormon prolaktin (PRL). Estradiol (E2) je hormon, katerega proizvodnja je uravnavana s strani hipofize in pripomore k nastanku prolaktinoma, ki je benigni tumor hipofize. Z L-DOPA zdravimo Parkinsonovo bolezen. L-DOPA je prekurzor dopamina, ki za kratek čas zviša njegov nivo, s tem zavira delovanje PRL in posledično nastanek prolaktinoma. Sinaptotagmin 4 (SYT4) je gen zgodnjega odgovora, ki sodeluje pri sproščanju veziklov. Namen naloge je bil raziskati, če je povečano izločanje PRL pri ovariektomiranih živalih z estradiolskimi vsadki povezano s spremembami v izražanju SYT4. Izražanje SYT4 in PRL smo analizirali z metodo imunohistokemije, svetlobnim mikroskopom, ter računalniško obdelavo posnetkov. Pri podganah, ki so prejemale E2, ne glede na prisotnost L-DOPA, je bilo sintetiziranega več SYT4 in manj PRL, v primerjavi z adenohipofizami živali kjer E2 ni bil prisoten in je bilo sintetiziranega manj SYT4, ter več PRL. V primerjavi s količinami PRL povprečnega odstotka površine na rezinah adenohipofiz, kjer je prišlo do izražanja PRL, so koncentracije PRL v krvnem obtoku podgan ravno obratne.

• Language: Slovenian
• Keywords: hipofiza, prolaktin, sinaptotagmin IV, estradiol, L-DOPA
• Work type: Master's thesis/paper
• Organization: BF - Biotechnical Faculty
• Year: 2018
• PID: 20.500.12556/RUL-105535
• COBISS.SI-ID: 4934479
• Publication date in RUL: 05.12.2018

Secondary language

• Language: English
• Title: The effect of estradiol and prolactin on expression of synaptoagmin IV in rat hypophysis

Abstract

Pituitary gland is an internal secretion gland that controls and regulates the functioning of other glands. It contains lactotrophs, hormone prolactin (PRL) secreting cells. Estradiol (E2) is a hormone whose production is regulated by the pituitary gland and contributes to the formation of prolactinoma, which is a benign tumour of the pituitary gland. L-DOPA is commonly used as a first line of treatment for Parkinson's disease. It is a dopamine precursor that increases levels of dopamine for a short period, thereby inhibiting the action of PRL and consequently the formation of prolactinoma. Synaptotagmin 4 (SYT4) is an early response gene, which is involved in the release of vesicles. The aim was to investigate whether the increase in PRL secretion in ovariectomized animals with E2 implants is associated with changes in the expression of SYT4. The expression of SYT4 and PRL was analysed using immunohistochemistry, light microscopy and computerized image processing. In rats, which were receiving E2, irrespective of the presence of L-DOPA, more SYT4 was synthesised and less PRL, compared to adenohypophyses of animals where E2 was not present; in those animals, less SYT4 and more PRL was synthesized. Compared with the PRL quantities measured by immunohistochemistry, the serum PRL concentrations showed the opposite result.

• Keywords: pituitary gland, prolactin, synaptotagmin IV, estradiol, L-DOPA