Vpliv kobiljega mleka na živost celic Caco-2

Založnik, Janja (author), Narat, Mojca (mentor), Potočnik, Klemen (comentor)

Trajna celična linija Caco-2 je zelo dobro poznan model človeškega črevesnega epitelija, ki se uporablja v številnih raziskavah za preizkušanje absorbcije različnih učinkovin, njihove presnove, porazdelitve in izločanja metabolitov. V zadnjih letih uživanje kobiljega mleka v humani prehrani močno narašča, saj mu pripisujejo številne, zdravju ugodne učinke. Kljub povečanemu zanimanju je kobilje mleko še dokaj neraziskano živilo, kajti na voljo je le omejen nabor eksperimentalno podprtih raziskav o učinkih kobiljega mleka na imunski sistem človeka. Ker kobilje mleko vse bolj pridobiva na pomenu tudi v širši javnosti, je bil glavni namen naloge preveriti ali lahko celice Caco-2 uporabljamo kot model za proučevanje učinkov kobiljega mleka. Na celičnem modelu smo proučevali kako različne koncentracije kobiljega mleka vplivajo na viabilnost celic Caco-2. Za izvedbo poskusa smo uporabili 5, 10, 20 in 40 % redčitve kobiljega mleka ter celice inkubirali v tako redčenem mleku 3, 6, 24, 48 in 72 ur. Viabilnost celic smo spremljali z metodo štetja celic po barvanju s tripan modrim barvilom. Na podlagi rezultatov smo ugotovili, da ima kobilje mleko negativen vpliv na viabilnost celic Caco-2. Z naraščanjem koncentracije kobiljega mleka je namreč viabilnost celic vidno upadala. Statistično značilne razlike v poskusu smo opredelili kot koncentracijsko ter časovno odvisne. Raziskava je pokazala, da se z visokimi koncentracijami kobiljega mleka tovrstnega poskusa ne da izvajati.

• Language: Slovenian
• Keywords: prehrana ljudi, kobilje mleko, imunologija, črevesje, celice Caco-2
• Work type: Master's thesis/paper
• Organization: BF - Biotechnical Faculty
• Year: 2018
• PID: 20.500.12556/RUL-105404
• COBISS.SI-ID: 4156808
• Publication date in RUL: 25.11.2018

Secondary language

• Language: English
• Title: The effect of mare's milk on viability of Caco-2 cells

Abstract

The continuous cell line Caco-2 is a well-known human intestinal epithelium model, which is used in several researches for testing the absorption of different active substances, their digestion, distribution, and release of metabolites. In the recent years, we have seen an increased consumption of mare’s milk in human diet, as the drink was linked to several health benefits. Despite this growing interest, mare’s milk remains relatively unresearched. In fact, there are only a few experimentally supported studies on the effects of mare’s milk on human immune system available. Seeing that mare’s milk is becoming increasingly important among the general public, this paper focuses on researching whether or not Caco-2 cells could be used as a model for studying the effects of mare’s milk. We used the cell model to study how different concentrations of mare’s milk affect the viability of Caco-2 cells. To perform the experiment, we used 5, 10, 20, and 40 % concentrations of mare’s milk, in which we incubated the cells for 3, 6, 24, 48, and 72 hours. We monitored the viability of cells with the method of trypan blue cell counting. Based on our findings, we learned that mare’s milk has a negative effect on the viability of Caco-2 cells. With the increase of concentration of mare’s milk, the viability of cells decreased visibly. The statistically significant differences in the experiment were classified as dependent on concentration and time. In conclusion, our research showed that this experiment cannot be performed with high concentrations of mare’s milk.

• Keywords: human nutrition, mare´s milk, immunology, intestines, cells Caco-2