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Vpliv zunajceličnih polimerov na mehanske in funkcionalne lastnosti biofilmov bakterije Escherichia coli
Horvat, Maruša (Author), Stopar, David (Mentor) More about this mentor... This link opens in a new window

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Abstract
Mehansko stabilnost biofilmu daje zunajcelični matriks, ki zapolni prostor med bakterijskimi celicami in jih drži skupaj. V magistrski nalogi smo testirali, kako sprememba izražanja zunajceličnih polimerov matriksa pri biofilmih bakterije E. coli (PGA, kodri, kolanska kislina), vpliva na viskoznost in viskoelastičnost biofilma, pa tudi, kako vpliva na odpornost proti protimikrobnim sredstvom, specifično proti vodikovemu peroksidu. Dodatno smo testirali, kako dodatek vode oziroma detergenta SDS vpliva na viskoznost posameznih biofilmov. Ugotovili smo, da se viskoznost in viskoelastičnost biofilmov z različnim izražanjem zunajceličnih polimerov značilno razlikuje. Največji efekt na mehanske lastnosti biofilma je imela odsotnost proteinskih kodrov. Prekomerno izražanje kolanske kisline je zmanjšalo viskoznost, pseudoplastičnost in stabilnost biofilma. Odsotnost sinteze PGA, kodrov in kolanske kisline ni zavrla nastanka biofilma. Dodatek vode ali raztopine SDS je značilno omehčal biofilme. Učinkovitost delovanja vodikovega peroksida na celice v biofilmu se med sevi razlikuje. Po dovzetnosti za vodikov peroksid izstopa mutanta MG1655 csrA, ki prekomerno izraža PGA, pri kateri je bil vodikov peroksid znatno bolj učinkovit kot pri ostalih sevih. Kljub dostopnosti za vodikov peroksid niso propadle vse celice na površini.

Language:Slovenian
Keywords:Escherichia coli, biofilmi, reološke lastnosti, viskoznost, viskoelastičnost, zunajcelični polimeri, vodikov peroksid
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2018
Publisher:[M. Horvat]
UDC:579.24/.26:579.842:544
COBISS.SI-ID:4974968 This link opens in a new window
Views:877
Downloads:451
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Secondary language

Language:English
Title:The effect of extracellular matrix polimers on mechanical and functional properties of Escherichia coli biofilms
Abstract:
Biofilm mechanical stability is due to extracellular matrix, which fills the space between bacterial cells and binds them together. In this work we tested, how different expression of extracellular polimers in matrix in E. coli biofilms (PGA, curli, colanic acid) effects biofilm viscosity and viscoelasticity, as well as how it effects the resistance agains hydrogen peroxide. Additionally we tested the effect of adding water or detergents on biofilm viscosity. Results showed significant differences in viscosity and viscoelasticity among biofilms with different expression of extracellular polimers. The absence of proteinaceus curli had the greatest effect on mechanical properties. Overproducing of colanic acid significantly decreased viscosity, pseudoplasticity and also biofilm stability. In the absence of PGA, curli and colanic acid production, E. coli could still form a biofilm. Adding water or SDS solution significantly softened the biofims. The effect of hydrogen peroxide on bacterial cells was different among biofilms. MG1655 csrA mutant, which is overproducing PGA, showed the greatest susceptibility for hydrogen peroxide. Not all the cells in biofilm were killed by hydrogen peroxide.

Keywords:Escherichia coli, biofilms, reologic properties, viscosity, viscoelasticity, extracellular polimers, hydrogen peroxide

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