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Vpliv transplantacije kostnega mozga pri miših na maso očesnih leč
ID Lončar, Maja (Author), ID Malovrh, Tadej (Mentor) More about this mentor... This link opens in a new window

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Abstract
Raziskovalci so že v 20. stoletju preučevali korelacijo med maso leče in starostjo organizma. Na podlagi tega smo maso leč uporabili tudi kot možno podlago za določanje vpliva transplantacije kostnega mozga na rast in staranje, ter s tem na maso očesnih leč pri miših. Namen raziskave je bil najprej validirati uporabljeno metodiko, ki vodi do verodostojnih rezultatov, med katerimi smo nato v programu SPSS opravili primerjalne statistične analize. Potrdili smo, da lahko zanemarimo vpliv časa fiksacije očesnih leč v formalinu na njihovo maso, in prav tako tudi, da vpliv vlage na suho maso očesnih leč ob standardiziranem sušenju, lahko izničimo. S tem smo zagotovili, da so naše metode ustrezne za bistveni del raziskav. Ugotovili smo, da masa leč s starostjo narašča in da se to naraščanje s starostjo miši upočasni. Potrdili smo tudi, da je masa miši v delni korelaciji z maso leče, pri čemer obstaja tudi povezava med starostjo in maso miši. Poleg tega smo s primerjavo mase očesnih leč kontrolne ter poskusne skupine miši s transplantiranim kostnim mozgom prikazali, da ni statistično pomembnih razlik v masi leč. Na podlagi rezultatov med drugim lahko zaključimo, da je masa leč uporabna indikatorska metoda za določanje starosti laboratorijskih miši, prav tako pa na podlagi danega modela transplantacije kostnega mozga lahko ovržemo tezo, da ima transplantacija lahko vpliv na maso očesnih leč pri miših.

Language:Slovenian
Keywords:biologija, oko, suha masa leče, mokra masa leče, transplantacija kostnega mozga, vpliv transplantacijena maso leče, vpliv formalina na maso leče, rast leče
Work type:Master's thesis/paper
Organization:BF - Biotechnical Faculty
Year:2018
PID:20.500.12556/RUL-105054 This link opens in a new window
COBISS.SI-ID:4929103 This link opens in a new window
Publication date in RUL:24.10.2018
Views:1340
Downloads:254
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Secondary language

Language:Unknown
Title:The influence of bone marrow transplantation on eye lens mass in mice
Abstract:
Already in the 20th century, researchers have been studying the correlation between the mass of the lens and the age of the body. In reference to this the mass of the lens was used as a basis for analyzing the effect of the bone marrow transplantation on growth and ageing, and thus to the weight of the ocular lenses of mice. The purpose of the research was therefore, to first validate the applied methodology, which led to credible results. The obtained results were then processed by comparative statistical analysis in the SPSS programme. The purpose of the research was therefore, to first validate the applied methodology, which led to credible results. The obtained results were then processed by comparative statistical analysis in the SPSS programme. We have confirmed that the fixation time of ocular lenses in formalin does not play a significant role (or: can be neglected). Furthermore, in the event of the standardized drying, the effect of moisture on the dry weight of the ocular lenses can be nullified. This ensured our methods as appropriate for the essential part of the research. We have found that with the age of the organism, the mass of lenses increases and also that the increase rate decelerates with the age of mice. We also confirmed that the mass of mice is in partial correlation with the weight of the lens, which means that there is also a link between the age and the weight of mice. In addition, comparing the masses of ocular lenses of control and experimental groups of mice with transplanted bone marrow showed that there are no statistically significant differences in the weight of the lenses. From the results it is to conclude that the mass of the lenses is a useful indicator for determining the age of laboratory mice. Based on the given model of bone marrow transplantation, the thesis that the bone marrow transplantation might have an impact on the weight of the eye lenses in mice is refuted.

Keywords:biology, eye, dry weight of ocular lens, wet weight of ocular lens, bone marrow transplantation, influence of bone marrow transplantation, influence of formalin fixation, lens growth

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