izpis_h1_title_alt

Lokalizacija encima ACC-oksidaze v plodovih paradižnika (Solanum lycopersicum)
Lavrič, Neja (Author), Strgulc Krajšek, Simona (Mentor) More about this mentor... This link opens in a new window, Kladnik, Aleš (Co-mentor)

URLURL - Presentation file, Visit http://pefprints.pef.uni-lj.si/5347/ This link opens in a new window

Abstract
Etilen je majhna plinska molekula, ki so jo raziskovalci odkrili v 19. stoletju in jo kasneje prepoznali kot prvi rastlinski hormon. Njena vloga je najbolj prepoznavna v biologiji rastlin, saj sodeluje v različnih procesih tekom celotnega življenjskega cikla rastline. Ti procesi vključujejo kalitev rastline, razvoj korenin in cvetov, zorenje plodov, senescenco, abscizijo in odgovore rastline tako na biotske (patogeni) kot na abiotske dejavnike (poškodba rastline, pomanjkanje kisika, mraz, poplave in suše). Sintetizira se iz prekurzorja 1-aminociklopropan-1-karboksilne kisline (ACC), njegovo pretvorbo pa katalizira encim ACC-oksidaza. ACC-oksidazo najdemo tako v vegetativnih, kakor v reproduktivnih tkivih rastline. Z raziskavo smo želeli ugotoviti, katera so specifična mesta, kjer se nahaja ACC-oksidaza v plodovih paradižnika. Encim smo lokalizirali z metodo imunolokalizacije z uporabo specifičnih primarnih protiteles proti ACC-oksidazi in raziskali, kako vpliva uporaba različnih pufrov v postopku povrnitve antigenov. Z imunolokalizacijo se obarvajo specifična mesta, kjer se nahaja ACC-oksidaza, predvsem na bazi ploda in v predelu žil v plodu ter v peclju ploda. Z uporabo različnih pufrov v postopku povrnitve antigenov smo opazili razlike v jakosti specifičnega obarvanja in količini nespecifičnega barvanja ozadja. Najmočnejši specifičen signal in hkrati najmanj nespecifično barvanje ozadja smo dobili z uporabo citratnega pufra pH 6 v postopku povrnitve antigenov. Kontrolne preparate smo barvali z uporabo neimunega seruma namesto z specifičnimi primarnimi protitelesi. V kontrolnih preparatih nismo zaznali specifičnega obarvanja.

Language:Slovenian
Keywords:ACC-oksidaza
Work type:Bachelor thesis/paper (mb11)
Tipology:2.11 - Undergraduate Thesis
Organization:PEF - Faculty of Education
Year:2018
COBISS.SI-ID:12138313 Link is opened in a new window
Views:500
Downloads:109
Metadata:XML RDF-CHPDL DC-XML DC-RDF
 
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Secondary language

Language:English
Title:Localization of the ACC-Oxidase enzyme in the tomato (Solanum lycopersicum)fruit
Abstract:
Ethylene is a small gaseous molecule that was discovered in the 19th century and was later recognized as the first plant hormone. It plays an important role in plant biology because it is involved in many aspects of the plant life cycle, including seed germination, root and flower development, fruit ripening, senescence, abscission and responses to biotic ( pathogen attack) and abiotic (wounding, hypoxia, chilling, floods and droughts) stresses. Ethylene is synthesized from 1-aminocyclopropane-1-carboxylic acid (ACC) where the reaction is catalyzed by the enzyme ACC oxidase. ACC oxidase is expressed in all vegetative and reproductive plant tissues. The aim of the study was to find out the location of ACC oxidase in the developing fruit of tomato. To localize the enzyme, we used immunolocalization with specific primary antibodies against ACC oxidase and examined the effect of different buffers in antigen retrieval. Using immunolocalization we detected ACC oxidase specifically inside the fruit and in the vascular region of the fruit and fruit pedicel. In the step of antigen retrieval during the immunolocalization process we used three different buffers that resulted in differences in the intensity of specific staining and the amount of nonspecific background staining. The strongest specific staining with the lowest background was achieved by using citrate buffer pH 6 in the step of antigen retrieval. Negative controls were stained using the nonimmune serum instead of specific primary antibodies. No specific staining was observed in the control sections.

Keywords:ACC-oxidase

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back