Vpliv sekundarnih metabolitov navadne konoplje (Canabis sativa L.) na kemotropični odziv entomopatogenih ogorčic (Steinernema in Heterorhabditis)

Cuderman, Tina (author), Laznik, Žiga (mentor), Flajšman, Marko (co-mentor)

Entomopatogene ogorčice (EPO) lahko uporabljamo kot enega od ukrepov v namen biotičnega varstva rastlin. V našem poskusu smo proučevali vpliv nekaterih etanolnih izvlečkov (EI) rastlinskih tkiv (listi, vršički, korenine) navadne konoplje na posamezne vrste EPO Steinernema carpocapsae, S. feltiae in Heterorhabditis bacteriophora oz. vpliv na njihove infektivne ličinke (IL). Predvidevali smo, da lahko na kemotropizem posamezne vrste EPO vplivajo različni dejavniki: vrsta EPO, vpliv izbrane EI, temperatura in način iskanja gostitelja. Poskus smo izvedli v laboratorijskih razmerah pri dveh temperaturah (20 in 25 °C) in 70 % relativni zračni vlagi. Gibanje posameznih IL smo preverjali po 24 urah. Na podlagi zbranih podatkov smo pozneje izračunali kemotropične indekse posameznih snovi in ugotovili, da nekatere EI lahko delujejo privabilno ali odvračalno. Na vrsto H. bacteriophora je EI FC pri 25 °C deloval privabilno. Na vrsto S. feltiae je EI FC pri 20 °C deloval odvračalno, pri 25 °C pa sta EI 11/37C in 707/10C delovala privabilno. Na vrsto S. carpocapsae sta EI TC in FC pri 20 °C delovala odvračalno, pri 25 °C pa je EI TL deloval privabilno. Na vrsto H. bacteriophora je EI FC pri 25 °C deloval privabilno. V našem poskusu se je za najbolj mobilno vrsto izkazala H. bacteriophora, za najmanj mobilno pa S. carpocapsae. Dokazali smo, da so bile EPO, ki svojega gostitelja iščejo aktivno, bolj mobilne kot tiste, ki imajo pasivno strategijo iskanja gostitelja. Tudi temperatura se je pokazala za pomemben dejavnik, saj je višja temperatura povzročila izrazito drugačno gibanje EPO, kar bi lahko prišteli vrstno specifični lastnosti EPO.

• Language: Slovenian
• Keywords: sekundarni metaboliti, navadna konoplja, kemotropični odziv, entomopatogene ogorčice
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [T. Cuderman]
• Year: 2018
• PID: 20.500.12556/RUL-104043
• UDC: 632.937.1:633.522:577.121(043.2)
• COBISS.SI-ID: 9051257
• Publication date in RUL: 03.10.2018

Secondary language

• Language: English
• Title: Influence of hemp (Cannabis sativa L.) secondary metabolites on the chemotropic response of enthomopathogenic nematodes (Steinernema and Heterorhabditis)

Abstract

Entomopathogenic nematodes (EPNs) can be used as one of the measures for the purpose of biological control. In our experiment we studied influence of some ethanol extracts (EE) of the hemp tissues (leaf, tips, roots) on the individual types of EPNs Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora. Our hypothesis was that different factors can have an effect on the chemotaxis of individual types of EPNs: species of EPNs, selected EE, temperature and the foraging strategy. Our experiment was carried out in Petri dishes, which contained agar at two different temperatures (20 and 25 °C) and 70 % relative humidity. The movement of infective juveniles (IJ) was observed after 24 hours. Based on our results we calculated the chemotaxis index of different EE. On the H. bacteriophora EE FC was at 25 °C very attractive. On the S. feltiae EE FC at 20 °C acted as repellent, EE 11/37C and 707/10C at 25 °C acted very attractive. On the S. carpocapsae EE TC and FC at 20 °C acted as repellent, at 25 °C EE TL was very attractive. On the H. bacteriophora EE FC was very attractive at 25 °C. In our experiment H. bacteriophora was the most mobile species, the least mobile species was S. carpocapsae. We proved that those EPNs which have an active foraging strategy are more mobile than those who have a passive foraging strategy. The temperature also turned out as an important factor, because higher temperature caused significantly different movement of the EPNs which could be one of the specific characteristics of EPNs.

• Keywords: secondary metabolites, hemp, chemotropic response, entomopathogenic nematodes