The main purpose of this dissertation is to represent different ways of valuing startup companies and all of the problems that arise. Such companies need to know their value so they can attract investors, but that is not easy to do considering lack of information that could help at valuing. They can be evaluated by classical methods, but they don't always give the true value. This happens due to the high risk that needs to be included in the value through individual parameters. Startup companies are on the rise nowdays, but most of them do not survive, so the risk of a company's downfall is high. In addition, there are some other factors that make it difficult to evaluate, such as: forecast of cash flows based on zero or very little historical data and determining the cost of capital. An alternative approach to evaluating startup companies is a real option method. The key advantage is that it involves managerial flexibility and helps to make the best decisions. Of course, this method also has some disadvantages, such as a complex mathematical structure, the model is too theoretical, it is difficult to determine the value of the underlying asset and the time of the end of the investment opportunity. This thesis also presents the evaluation of a concrete startup company, which shows that real options really do have a positive impact on the value of the company.