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Tkivne kulture navadne konoplje (Canabis sativa L.): adventivna regeneracija in mikropropagacija
Mestinšek Mubi, Špela (Author), Murovec, Jana (Mentor) More about this mentor... This link opens in a new window, Flajšman, Marko (Co-mentor)

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Abstract
Mikropropagacija je tehnika hitrega klonskega razmnoževanja v in vitro razmerah, ki omogoča visoko frekvenco razmnoževanja rastlin. Adventivna regeneracija pa je tehnika, kjer se iz ne-meristematskega tkiva regenerira nova rastlina. Cilji magistrske naloge so bili vzpostavitev protokola za sterilizacijo semen, vzpostavitev in vitro kulture poganjkov, uspešna mikropropagacija in regeneracija rastlin konoplje iz različnih rastlinskih izsečkov (hipokotilov in kotiledonov), preizkusiti uporabnost ISSR markerjev za ugotavljanje pojava somaklonske variabilnosti ter preveriti veljavnost postavljenih hipotez. V poskusih smo uporabili že poznana gojišča ter preizkusili nova gojišča za iniciacijo poganjkov ter za koreninjenje rastlin. Rezultati so pokazali, da se največ stranjskih poganjkov tvori na MS gojišču z dodanim citokininom TDZ, pri čemer so se za najboljšo iniciacijo kulture poganjkov izkazali tretji in četrti nodiji na rastlini, apikalni poganjki pa so bili najmanj primerni. Koreninjenje je bilo najuspešnejše na gojišču z dodanim citokininom meta-topolinom. Adventivna regeneracija rastlin je bila sortno pogojena in najuspešnejša iz rastlinskih izsečkov kotiledonov. Preizkušeni ISSR markerji so se pokazali kot uporabne za genetsko razlikovanje med različnimi genotipi navadne konoplje.

Language:Slovenian
Keywords:Cannabis sativa, industrijska konoplja, medicinska konoplja, mikropropagacija, adventivna regeneracija, ISSR markerji
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2018
Publisher:[Š. Mestinšek Mubi]
UDC:633.522:57.085:631.532(043.2)
COBISS.SI-ID:9020281 Link is opened in a new window
Views:487
Downloads:215
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Secondary language

Language:English
Title:Tissue culture of hemp (Cannabis sativa L.): Adventitious regeneration and micropropagation
Abstract:
Micropropagation is a technique of rapid in vitro clonal propagation of plants, which enables their high frequency multiplication. Adventitious regeneration is a process of in vitro production of new plant tissues and organs from non-meristematic cells. The aims of the master thesis were to develop protocols for sterilization of cannabis seeds, establishment of aseptic cultures, micropropagation and adventitious regeneration from different explants (hypocotyl and cotyledon), to test the usefulness of ISSR markers for detection of somaclonal variation and to check our hypotheses. In the experiments, we used already known plant tissue culture media and we tested some new media formulations for axillary shoots and roots initiation of plants. The best medium for the formation of the axillary shoots was MS basal medium supplemented with the hormone cytokinine TDZ and the longest shoots were initiated from the third and fourth nodes on the plant, while the apical shoots were less suitable for initiation of an in vitro shoot culture. MS basal medium supplemented with hormone cytokinine meta-topolin proved to be most suitable for rooting. In vitro regeneration of hemp is genotype dependent and the cotyledon explants proved to be more regenerative than hypocotyl explants. Our results with ISSR markers showed that they are useful for genetic differentiation between different genotypes of hemp.

Keywords:Cannabis sativa, industrial hemp, medical hemp, micropropagation, adventitious regeneration, ISSR markers

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