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Protimikrobno delovanje etanolnega izvlečka cvetov, listov in stebel rastline Anaphalis margaritacea na bakterije vrste Campylobacter jejuni
Kunčič, Ajda (Author), Smole Možina, Sonja (Mentor) More about this mentor... This link opens in a new window

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Abstract
Glede na prilagoditve bakterij, bodisi zaradi upravičene ali neupravičene prekomerne uporabe antibiotikov, se učinkovitost le-teh zmanjšuje, mikroorganizmi pa se bodo po vsej verjetnosti stalno prilagajali novim protimikrobnim snovem. Velik problem predstavljajo tudi bakterijske okužbe, ki jih povzroča vrsta Campylobacter jejuni. Zaradi pridobivanja odpornosti na antibiotike smo v diplomskem delu želeli preveriti protimikrobno delovanje rastlinskih izvlečkov cvetov, listov in stebel rastline Anaphalis margaritacea na bakterijski sev Campylobacter jejuni NCTC 11168. Najprej smo pripravili etanolni izvleček cvetov, listov in stebel rastline Anaphalis margaritacea in z UHPLC-PDA-ESI-MS analizo določili prisotnost različnih fenolnih spojin v izvlečkih. Potem smo s poskusom določili minimalno inhibitorno koncentracijo (MIK) in minimalno bakteriocidno koncentracijo (MBK) etanolnih izvlečkov. MIK in MBK etanolnega izvlečka cvetov in listov je bila 500 µg/mL, MIK in MBK etanolnega izvlečka stebel pa 1000 µg/mL. Glede na dobljene rezultate, smo preverili še vpliv etanolnih izvlečkov na membranske izlivne črpalke seva Campylobacter jejuni NCTC 11168, pri čemer smo uporabili sub-inhibitorne koncentracije, in sicer ½ in ¼ MIK. Rezultati so pokazali boljši vpliv etanolnega izvlečka cvetov v koncentraciji ¼ MIK (125 µg/mL) in listov v koncentraciji ½ in ¼ MIK (250 µg/mL in 125 µg/mL) na membranske izlivne črpalke kot etanolnega izvlečka stebel v koncentraciji ½ in ¼ MIK (500 µg/mL in 250 µg/mL). Pri koncentraciji ½ MIK (250 µg/mL) etanolnega izvlečka cvetov sklepamo, da uporabljena sub-inhibitorna koncentracija že zavira rast bakterijskega seva Campylobacter jejuni NCTC 11168.

Language:Slovenian
Keywords:patogene bakterije, Campylobacter jejuni, rastlinski izvlečki, Anaphalis margaritacea, protimikrobne snovi, fenolne spojine, protimikrobno delovanje, membranske izlivne črpalke
Work type:Bachelor thesis/paper (mb11)
Tipology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2018
Publisher:[A. Kunčič]
UDC:579.24/.26:547.9+547.56
COBISS.SI-ID:4928376 Link is opened in a new window
Views:244
Downloads:103
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Secondary language

Language:English
Title:Antimicrobial activity of the ethanol extracts from flowers, leaves and stems of Anaphalis margaritacea against Campylobacter jejuni
Abstract:
Regarding the adaptation of bacteria, either because of justified or unjustified excessive use of antibiotics, the efficacy of these decreases and microorganisms are likely to be constantly adapting to new antimicrobial agents. Campylobacteriosis caused by Campylobacter jejuni can cause many problems. Due to the acquisition of antibiotic resistance in our thesis we wanted to check the antimicrobial activity of plant extracts from flowers, leaves and stems of Anaphalis margaritacea on the bacterial Campylobacter jejuni NCTC 11168 strain. We first prepared ethanol extract from flowers, leaves and stems of Anaphalis margaritacea and with UHPLC-PDA-ESI-MS determined the presence of various phenolic compounds in the extracts. Then we determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the ethanol extracts. MIC and MBC of ethanol extract from flowers and leaves was 500 µg/mL, while MIC and MBC of ethanol extract from stems was 1000 µg/mL. According to the results, we have checked the influence of the ethanol extracts on membrane efflux pumps of Campylobacter jejuni NCTC 11168 strain, where we used the sub-inhibitory concentrations corresponding to ½ and ¼ MIC. The results showed a better effect of the ethanol extract from the flowers in a concentration corresponding to ¼ MIC (125 µg/mL) and leaves in a concentrations of ½ and ¼ MIC (250 µg/mL and 125 µg/mL) on the membrane efflux pumps, as the ethanol extract from the stems in a concentrations of ½ and ¼ MIC (500 µg/mL in 250 µg/mL). At a concentration corresponding to ½ MIC (250 µg/mL) of the ethanol extract from flowers we conclude that the used sub-inhibitory concentration already inhibits the growth of a bacterial strain Campylobacter jejuni NCTC 11168.

Keywords:pathogens, Campylobacter jejuni, plant extracts, Anaphalis margaritacea, antimicrobial compounds, phenolic compounds, antimicrobial activity, efflux pump

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