The goal of this research was the isolation of nanocellulose from juvenile and adult wood of Norway spruce. The process was done first by mechanically sewing and grinding the wood to make wood dust. The next step was the chemical treatment by organic solvents in order to remove polar and non-polar extractives from the wood dust. Next two steps were delignification and the removal of hemicellulose, the result of which was [alpha]-cellulose. Then we used TEMPO oxidation and mechanical processing for the disintegration of the cellulose fibers. Next two steps were TEMPO oxidation and mechanical treatment. This way we were able to acquire a substance that contained cellulose fibers, fragments of cellulose fibers, fragments of cell walls and small particles that formed a gel. This gel was similar to the type of gel that is characteristic to the one formed by nanocellulose. The process that was used can be drastically improved by a better selection of input material, finer grinding and more effective chemical and mechanical treatment.
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