Impact of mycotoxin metabolites deepoxy-deoxynivalenol and beta-zearalenol on bovine preimplantation embryo development in the presence of acetonitrile

Gačnikar, Jernej (avtor), Mrkun, Janko (avtor), Babič, Janja (avtor), Sterniša, Meta (avtor), Zakošek, Maja (avtor)

The quality of animal feed is increasingly affected by weather conditions, high humidity, and damage to grains, which have led to various mycotoxin-producing moulds. The aim of this study was to determine the effects of the combination of deepoxy-deoxynivalenol and beta-zearalenol on the development of preimplantation bovine embryos, the extent to which the presence of both mycotoxin metabolites affects the development of in vitro cultured bovine embryos, or whether the effect of both toxins enhances embryotoxicity. Ovaries were transported from the abattoir to the laboratory and, after maturation and fertilisation, zygotes were placed in an embryo culture medium (IVC) with different mycotoxin metabolite concentrations diluted in acetonitrile. It was found that the blastocyst rate of cleaved embryos was affected by 1 μL acetonitrile in 400 μL medium (0.25%) compared to the group without acetonitrile. For this reason, it was decided to use acetonitrile as a control group, and the desired mycotoxin metabolite concentrations were diluted in the lowest possible amount of acetonitrile (0.5 μL) that could be accurately added to the study groups. There was no statistical difference when the higher mycotoxin metabolite concentrations were added.

• Jezik: Angleški jezik
• Ključne besede: mycotoxins, humidity, fertility, cattle, in vitro production, deepoxy-deoxynivalenol, beta-zearalenol, acetonitrile
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta ; BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 13 str.
• Številčenje: Vol. 11, iss. 6, art. 267
• PID: 20.500.12556/RUL-158471
• UDK: 617:618.1:636.09
• ISSN pri članku: 2306-7381
• DOI: 10.3390/vetsci11060267
• COBISS.SI-ID: 198787075
• Datum objave v RUL: 13.06.2024

GAČNIKAR, Jernej, MRKUN, Janko, BABIČ, Janja, STERNIŠA, Meta in ZAKOŠEK, Maja, 2024, Impact of mycotoxin metabolites deepoxy-deoxynivalenol and beta-zearalenol on bovine preimplantation embryo development in the presence of acetonitrile. Veterinary sciences [na spletu]. 2024. Vol. 11, no. 6,  267. [Dostopano 7 april 2025]. DOI 10.3390/vetsci11060267. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=158471

Gradivo je del revije

• Naslov: Veterinary sciences
• Skrajšan naslov: Vet. sci.
• Založnik: MDPI
• ISSN: 2306-7381
• COBISS.SI-ID: 523429657

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikotoksini, reprodukcija, in vitro, deepoksi-deoksinivalenol, beta-zearalenol, acetonitril

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0053
• Naslov: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Program financ.: Young researchers