Impact of mycotoxin metabolites deepoxy-deoxynivalenol and beta-zearalenol on bovine preimplantation embryo development in the presence of acetonitrile

Gačnikar, Jernej (author), Mrkun, Janko (author), Babič, Janja (author), Sterniša, Meta (author), Zakošek, Maja (author)

The quality of animal feed is increasingly affected by weather conditions, high humidity, and damage to grains, which have led to various mycotoxin-producing moulds. The aim of this study was to determine the effects of the combination of deepoxy-deoxynivalenol and beta-zearalenol on the development of preimplantation bovine embryos, the extent to which the presence of both mycotoxin metabolites affects the development of in vitro cultured bovine embryos, or whether the effect of both toxins enhances embryotoxicity. Ovaries were transported from the abattoir to the laboratory and, after maturation and fertilisation, zygotes were placed in an embryo culture medium (IVC) with different mycotoxin metabolite concentrations diluted in acetonitrile. It was found that the blastocyst rate of cleaved embryos was affected by 1 μL acetonitrile in 400 μL medium (0.25%) compared to the group without acetonitrile. For this reason, it was decided to use acetonitrile as a control group, and the desired mycotoxin metabolite concentrations were diluted in the lowest possible amount of acetonitrile (0.5 μL) that could be accurately added to the study groups. There was no statistical difference when the higher mycotoxin metabolite concentrations were added.

• Language: English
• Keywords: mycotoxins, humidity, fertility, cattle, in vitro production, deepoxy-deoxynivalenol, beta-zearalenol, acetonitrile
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty; BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 13 str.
• Numbering: Vol. 11, iss. 6, art. 267
• PID: 20.500.12556/RUL-158471
• UDC: 617:618.1:636.09
• ISSN on article: 2306-7381
• DOI: 10.3390/vetsci11060267
• COBISS.SI-ID: 198787075
• Publication date in RUL: 13.06.2024

GAČNIKAR, Jernej, MRKUN, Janko, BABIČ, Janja, STERNIŠA, Meta and ZAKOŠEK, Maja, 2024, Impact of mycotoxin metabolites deepoxy-deoxynivalenol and beta-zearalenol on bovine preimplantation embryo development in the presence of acetonitrile. Veterinary sciences [online]. 2024. Vol. 11, no. 6,  267. [Accessed 11 April 2025]. DOI 10.3390/vetsci11060267. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=158471

Record is a part of a journal

• Title: Veterinary sciences
• Shortened title: Vet. sci.
• Publisher: MDPI
• ISSN: 2306-7381
• COBISS.SI-ID: 523429657

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: mikotoksini, reprodukcija, in vitro, deepoksi-deoksinivalenol, beta-zearalenol, acetonitril

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P4-0053
• Name: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

• Funder: ARIS - Slovenian Research and Innovation Agency
• Funding programme: Young researchers