Comparing the effects of encapsulated and non-encapsulated propolis extracts on model lipid membranes and lactic bacteria, with emphasis on the synergistic effects of its various compounds

Šturm, Luka (avtor), Osojnik Črnivec, Ilja Gasan (avtor), Prislan, Iztok (avtor), Poklar Ulrih, Nataša (avtor)

Propolis is a resinous compound made by bees with well-known biological activity. However, comparisons between encapsulated and non-encapsulated propolis are lacking. Therefore, the antibacterial activity, effect on the phase transition of lipids, and inhibition of UV-induced lipid oxidation of the two forms of propolis were compared. The results showed that non-encapsulated propolis produces quicker effects, thus being better suited when more immediate effects are required (e.g., antibacterial activity). In order to gain an in-depth introspective on these effects, we further studied the synergistic effect of propolis compounds on the integrity of lipid membranes. The knowledge of component synergism is important for the understanding of effective propolis pathways and for the perspective of modes of action of synergism between different polyphenols in various extracts. Thus, five representative molecules, all previously isolated from propolis (chrysin, quercetin, trans-ferulic acid, caffeic acid, (-)-epigallocatechin-3-gallate) were mixed, and their synergistic effects on lipid bilayers were investigated, mainly using DSC. The results showed that some compounds (quercetin, chrysin) exhibit synergism, whereas others (caffeic acid, t-ferulic acid) do not show any such effects. The results also showed that the synergistic effects of mixtures composed from several different compounds are extremely complex to study, and that their prediction requires further modeling approaches.

• Jezik: Angleški jezik
• Ključne besede: liposomes, lipid phase transition, chrysin, quercetin, (-)-epigallocatechin-3-gallate, transferulic acid, caffeic acid, UV-oxidation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 21 str.
• Številčenje: Vol. 28, iss. 2
• PID: 20.500.12556/RUL-145899
• UDK: 577.352:638.135:547.56
• ISSN pri članku: 1420-3049
• DOI: 10.3390/molecules28020712
• COBISS.SI-ID: 137197827
• Datum objave v RUL: 17.05.2023

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: propolis, kapsulacija, modelne lipidne membrane, fenolne spojine, epigalokatehin, epigalokatehin-3-galat, interakcije

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0121
• Naslov: Biokemijska in biofizikalno-kemijska karakterizacija naravnih snovi

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J2-3040
• Naslov: S posnemanjem endogenih lipidnih delcev do magnetno-odzivnih nanostruktur za izboljšanodostavo zdravil in nanodelcev