Comparing the effects of encapsulated and non-encapsulated propolis extracts on model lipid membranes and lactic bacteria, with emphasis on the synergistic effects of its various compounds

Šturm, Luka (author), Osojnik Črnivec, Ilja Gasan (author), Prislan, Iztok (author), Poklar Ulrih, Nataša (author)

Propolis is a resinous compound made by bees with well-known biological activity. However, comparisons between encapsulated and non-encapsulated propolis are lacking. Therefore, the antibacterial activity, effect on the phase transition of lipids, and inhibition of UV-induced lipid oxidation of the two forms of propolis were compared. The results showed that non-encapsulated propolis produces quicker effects, thus being better suited when more immediate effects are required (e.g., antibacterial activity). In order to gain an in-depth introspective on these effects, we further studied the synergistic effect of propolis compounds on the integrity of lipid membranes. The knowledge of component synergism is important for the understanding of effective propolis pathways and for the perspective of modes of action of synergism between different polyphenols in various extracts. Thus, five representative molecules, all previously isolated from propolis (chrysin, quercetin, trans-ferulic acid, caffeic acid, (-)-epigallocatechin-3-gallate) were mixed, and their synergistic effects on lipid bilayers were investigated, mainly using DSC. The results showed that some compounds (quercetin, chrysin) exhibit synergism, whereas others (caffeic acid, t-ferulic acid) do not show any such effects. The results also showed that the synergistic effects of mixtures composed from several different compounds are extremely complex to study, and that their prediction requires further modeling approaches.

• Language: English
• Keywords: liposomes, lipid phase transition, chrysin, quercetin, (-)-epigallocatechin-3-gallate, transferulic acid, caffeic acid, UV-oxidation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 21 str.
• Numbering: Vol. 28, iss. 2
• PID: 20.500.12556/RUL-145899
• UDC: 577.352:638.135:547.56
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules28020712
• COBISS.SI-ID: 137197827
• Publication date in RUL: 17.05.2023

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: propolis, kapsulacija, modelne lipidne membrane, fenolne spojine, epigalokatehin, epigalokatehin-3-galat, interakcije

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0121
• Name: Biokemijska in biofizikalno-kemijska karakterizacija naravnih snovi

• Funder: ARRS - Slovenian Research Agency
• Project number: J2-3040
• Name: S posnemanjem endogenih lipidnih delcev do magnetno-odzivnih nanostruktur za izboljšanodostavo zdravil in nanodelcev