Fast and simple LC-MS/MS method for rifampicin quantification in human plasma

Temova Rakuša, Žane (avtor), Roškar, Robert (avtor), Klančar, Anita (avtor), Trdan Lušin, Tina (avtor), Faganeli, Nataša (avtor), Grabnar, Iztok (avtor), Mrhar, Aleš (avtor), Kristl, Albin (avtor), Trontelj, Jurij (avtor)

A simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated. The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate. Chromatographic separation was achieved on an 1290 Infinity liquid chromatograph coupled to 6460 Triple Quadrupole operated in positive mode on a core-shell Kinetex C18 column (50 % 2.1 mm, 2.6 %m) by gradient elution using 0.1% formic acid in water and acetonitrile as a mobile phase. The proposed method is the fastest method published by now, both in terms of sample preparation (approximately one minute per sample) and chromatographic analysis (total run time 2.4 min). Another key benefit is the outstanding sensitivity and wide analytical range (5-40000 %g/L) with good linearity, accuracy, and precision. The method showed almost complete recovery (92%) and absence of any significant matrix effect as demonstrated by uniform responses from QC samples prepared in blood plasma from 6 volunteers (RSD <5%). The proposed method was successfully applied to rifampicin quantification in 340 patients' plasma samples, thus demonstrating its suitability for both therapeutic drug monitoring and pharmacokinetic analysis.

• Jezik: Angleški jezik
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: Str. 1-7
• Številčenje: art. 4848236, Vol. 2019
• PID: 20.500.12556/RUL-141359
• UDK: 543.544:616-002.5
• ISSN pri članku: 1687-8779
• DOI: 10.1155/2019/4848236
• COBISS.SI-ID: 4670321
• Datum objave v RUL: 29.09.2022

Gradivo je del revije

• Naslov: International Journal of Analytical Chemistry (Online)
• Skrajšan naslov: Int. J. Anal. Chem. (Online)
• Založnik: Hindawi Publishing Corporation
• ISSN: 1687-8779
• COBISS.SI-ID: 522084377

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Angleški jezik
• Ključne besede: rifampicin, človeška plazma, kromatografska separacija

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0189-2018
• Naslov: Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih