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Application of dynamic and static light scattering for size and shape characterization of small extracellular nanoparticles in plasma and ascites of ovarian cancer patients
ID
Kogej, Ksenija
(
Avtor
),
ID
Božič, Darja
(
Avtor
),
ID
Kobal, Borut
(
Avtor
),
ID
Herzog, Maruša
(
Avtor
),
ID
Černe, Katarina
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,98 MB)
MD5: EFF6385EC5E5607BF21EBAEB90F17741
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/1422-0067/22/23/12946
Galerija slik
Izvleček
In parallel to medical treatment of ovarian cancer, methods for the early detection of cancer tumors are being sought. In this contribution, the use of non-invasive static (SLS) and dynamic light scattering (DLS) for the characterization of extracellular nanoparticles (ENPs) in body fluids of advanced serous ovarian cancer (OC) and benign gynecological pathology (BP) patients is demonstrated and critically evaluated. Samples of plasma and ascites (OC patients) or plasma, peritoneal fluid, and peritoneal washing (BP patients) were analyzed. The hydrodynamic radius (R$_h$) and the radius of gyration (R$_g$) of ENPs were calculated from the angular dependency of LS intensity for two ENP subpopulations. R$_h$ and R$_g$ of the predominant ENP population of OC patients were in the range 20–30 nm (diameter 40–60 nm). In thawed samples, larger particles (R$_h$ mostly above 100 nm) were detected as well. The shape parameter ρ of both particle populations was around 1, which is typical for spherical particles with mass concentrated on the rim, as in vesicles. The R$_h$ and R$_g$ of ENPs in BP patients were larger than in OC patients, with ρ ≈ 1.1–2, implying a more elongated/distorted shape. These results show that SLS and DLS are promising methods for the analysis of morphological features of ENPs and have the potential to discriminate between OC and BP patients. However, further development of the methodology is required.
Jezik:
Angleški jezik
Ključne besede:
extracellular vesicles
,
extracellular nanoparticles
,
ovarian cancer
,
dynamic light scattering
,
static light scattering
,
particle size
,
particle shape
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FKKT - Fakulteta za kemijo in kemijsko tehnologijo
ZF - Zdravstvena fakulteta
MF - Medicinska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2021
Št. strani:
23 str.
Številčenje:
Vol. 22, iss. 23, art. 12946
PID:
20.500.12556/RUL-136697
UDK:
618.1
ISSN pri članku:
1422-0067
DOI:
10.3390/ijms222312946
COBISS.SI-ID:
87267843
Datum objave v RUL:
16.05.2022
Število ogledov:
770
Število prenosov:
152
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
International journal of molecular sciences
Skrajšan naslov:
Int. j. mol. sci.
Založnik:
MDPI
ISSN:
1422-0067
COBISS.SI-ID:
2779162
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
01.12.2021
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
ekstracelularni vezikli
,
zunajcelični nanodelci
,
rak jajčnikov
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P1-0201
Naslov:
Fizikalna kemija
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P3-0067
Naslov:
Farmakologija in farmakogenomika
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
University Medical Centre Ljubljana
Številka projekta:
20160084
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