Application of dynamic and static light scattering for size and shape characterization of small extracellular nanoparticles in plasma and ascites of ovarian cancer patients

Kogej, Ksenija (avtor), Božič, Darja (avtor), Kobal, Borut (avtor), Herzog, Maruša (avtor), Černe, Katarina (avtor)

In parallel to medical treatment of ovarian cancer, methods for the early detection of cancer tumors are being sought. In this contribution, the use of non-invasive static (SLS) and dynamic light scattering (DLS) for the characterization of extracellular nanoparticles (ENPs) in body fluids of advanced serous ovarian cancer (OC) and benign gynecological pathology (BP) patients is demonstrated and critically evaluated. Samples of plasma and ascites (OC patients) or plasma, peritoneal fluid, and peritoneal washing (BP patients) were analyzed. The hydrodynamic radius (R$_h$) and the radius of gyration (R$_g$) of ENPs were calculated from the angular dependency of LS intensity for two ENP subpopulations. R$_h$ and R$_g$ of the predominant ENP population of OC patients were in the range 20–30 nm (diameter 40–60 nm). In thawed samples, larger particles (R$_h$ mostly above 100 nm) were detected as well. The shape parameter ρ of both particle populations was around 1, which is typical for spherical particles with mass concentrated on the rim, as in vesicles. The R$_h$ and R$_g$ of ENPs in BP patients were larger than in OC patients, with ρ ≈ 1.1–2, implying a more elongated/distorted shape. These results show that SLS and DLS are promising methods for the analysis of morphological features of ENPs and have the potential to discriminate between OC and BP patients. However, further development of the methodology is required.

• Jezik: Angleški jezik
• Ključne besede: extracellular vesicles, extracellular nanoparticles, ovarian cancer, dynamic light scattering, static light scattering, particle size, particle shape
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo ; ZF - Zdravstvena fakulteta ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 23 str.
• Številčenje: Vol. 22, iss. 23, art. 12946
• PID: 20.500.12556/RUL-136697
• UDK: 618.1
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms222312946
• COBISS.SI-ID: 87267843
• Datum objave v RUL: 16.05.2022

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.12.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ekstracelularni vezikli, zunajcelični nanodelci, rak jajčnikov