Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor

Menegatti, Tadej (avtor), Žnidaršič Plazl, Polona (avtor)

A microbioreactor was developed in which selected amine transaminase was immobilized together with the cofactor pyridoxal phosphate (PLP) to allow efficient continuous transamination. The enzyme and cofactor were retained in a porous copolymeric hydrogel matrix formed in a two-plate microreactor with an immobilization efficiency of over 97%. After 10 days of continuous operation, 92% of the initial productivity was retained and no leaching of PLP or enzyme from the hydrogel was observed. The microbioreactor with co-immobilized cofactor showed similar performance with and without the addition of exogenous PLP, suggesting that the addition of PLP is not required during the process. The space-time yield of the microbioreactor was 19.91 g L−1 h−1, while the highest achieved biocatalyst productivity was 5.4 mg mgenzyme−1 h−1. The immobilized enzyme also showed better stability over a wider pH and temperature range than the free enzyme. Considering the time and cost efficiency of the immobilization process and the possibility of capacity expansion, such a system is of great potential for industrial application.

• Jezik: Angleški jezik
• Ključne besede: cofactor co-immobilization, hydrogel, microreactor, amine transaminase, continuous process
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Datum objave: 04.11.2021
• Leto izida: 2021
• Št. strani: Str. 1-10
• Številčenje: Vol. 9
• PID: 20.500.12556/RUL-136179
• UDK: 66.098
• ISSN pri članku: 2296-4185
• DOI: 10.3389/fbioe.2021.752064
• COBISS.SI-ID: 83665411
• Datum objave v RUL: 15.04.2022

Gradivo je del revije

• Naslov: Frontiers in bioengineering and biotechnology
• Skrajšan naslov: Front. bioeng. biotechnol.
• Založnik: Frontiers Media
• ISSN: 2296-4185
• COBISS.SI-ID: 523093017

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 04.11.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: koimobilizacija kofaktorja, hidrogel, mikroreaktor, amin transaminaza, kontinuirni procesi