Novel spray dried glycerol 2-phosphate cross-linked chitosan microparticulate vaginal delivery system-development, characterization and cytotoxicity studies

Szymańska, Emilia (avtor), Szekalska, M. (avtor), Czarnomysy, Robert (avtor), Lavrič, Zoran (avtor), Srčič, Stanko (avtor), Miltyk, Wojciech (avtor), Winnicka, K. (avtor)

Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using glycerol 2-phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of glycerol 2-phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the glycerol 2-phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.

• Jezik: Angleški jezik
• Ključne besede: chitosan, microparticles, glycerol 2-phosphate, spray drying, mucoadhesiveness, cytotoxicity
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2016
• Št. strani: 22 str.
• Številčenje: Vol. 14, iss. 10, art. 174
• PID: 20.500.12556/RUL-131509
• UDK: 577.1
• ISSN pri članku: 1660-3397
• DOI: 10.3390/md14100174
• COBISS.SI-ID: 4190065
• Datum objave v RUL: 29.09.2021

Gradivo je del revije

• Naslov: Marine drugs
• Skrajšan naslov: Mar. drugs
• Založnik: MDPI
• ISSN: 1660-3397
• COBISS.SI-ID: 23578841

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.10.2016

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: hitosan, mikrodelci, citotoksičnost, dostavni sistem, glicerol 2-fosfat

Projekti

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: Medical University of Białystok
• Številka projekta: N/ST/ZB/16/006/2215