Novel spray dried glycerol 2-phosphate cross-linked chitosan microparticulate vaginal delivery system-development, characterization and cytotoxicity studies

Szymańska, Emilia (author), Szekalska, M. (author), Czarnomysy, Robert (author), Lavrič, Zoran (author), Srčič, Stanko (author), Miltyk, Wojciech (author), Winnicka, K. (author)

Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using glycerol 2-phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of glycerol 2-phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the glycerol 2-phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.

• Language: English
• Keywords: chitosan, microparticles, glycerol 2-phosphate, spray drying, mucoadhesiveness, cytotoxicity
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2016
• Number of pages: 22 str.
• Numbering: Vol. 14, iss. 10, art. 174
• PID: 20.500.12556/RUL-131509
• UDC: 577.1
• ISSN on article: 1660-3397
• DOI: 10.3390/md14100174
• COBISS.SI-ID: 4190065
• Publication date in RUL: 29.09.2021

Record is a part of a journal

• Title: Marine drugs
• Shortened title: Mar. drugs
• Publisher: MDPI
• ISSN: 1660-3397
• COBISS.SI-ID: 23578841

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.10.2016

Secondary language

• Language: Slovenian
• Keywords: hitosan, mikrodelci, citotoksičnost, dostavni sistem, glicerol 2-fosfat

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: Medical University of Białystok
• Project number: N/ST/ZB/16/006/2215