Magnetic resonance imaging for rapid screening for the nephrotoxic and hepatotoxic effects of microcystins

Milutinović Živin, Aleksandra (avtor), Zorc-Pleskovič, Ruda (avtor), Živin, Marko (avtor), Vovk, Andrej (avtor), Serša, Igor (avtor), Šuput, Dušan (avtor)

In vivo visualization of kidney and liver damage by Magnetic Resonance Imaging (MRI) may offer an advantage when there is a need for a simple, non-invasive and rapid method for screening of the effects of potential nephrotoxic and hepatotoxic substances in chronic experiments. Here, we used MRI for monitoring chronic intoxication with microcystins (MCs) in rat. Male adult Wistar rats were treated every other day for eight months, either with MC-LR (10 μg/kg i.p.) or MC-YR (10 μg/kg i.p.). Control groups were treated with vehicle solutions. T$_1$-weighted MR-images were acquired before and at the end of the eight months experimental period. Kidney injury induced by the MCs presented with the increased intensity of T$_1$-weighted MR-signal of the kidneys and liver as compared to these organs from the control animals treated for eight months, either with the vehicle solution or with saline. The intensification of the T$_1$-weighted MR-signal correlated with the increased volume density of heavily injured tubuli (R$^2$ = 0.77), with heavily damaged glomeruli (R$^2$ = 0.84) and with volume density of connective tissue (R$^2$ = 0.72). The changes in the MR signal intensity probably reflect the presence of an abundant proteinaceous material within the dilated nephrons and proliferation of the connective tissue. T$_1$-weighted MRI-is a valuable method for the in vivo screening of kidney and liver damage in rat models of intoxication with hepatotoxic and nephrotoxic agents, such as microcystins.

• Jezik: Angleški jezik
• Ključne besede: microcystin, chronic toxicity, magnetic resonance imaging, kidney, liver, nephrotoxic
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2013
• Št. strani: Str. 2785-2798
• Številčenje: Vol. 11, iss. 8
• PID: 20.500.12556/RUL-129250
• UDK: 577.1
• ISSN pri članku: 1660-3397
• DOI: 10.3390/md11082785
• COBISS.SI-ID: 30744025
• Datum objave v RUL: 31.08.2021

Gradivo je del revije

• Naslov: Marine drugs
• Skrajšan naslov: Mar. drugs
• Založnik: MDPI
• ISSN: 1660-3397
• COBISS.SI-ID: 23578841

Licence

• Licenca: CC BY 3.0, Creative Commons Priznanje avtorstva 3.0 Nedoločena
• Povezava: https://creativecommons.org/licenses/by/3.0/deed.sl
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• Začetek licenciranja: 05.08.2013

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikrocistin, kronična toksičnost, slikanje z magnetno resonanco

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0019
• Naslov: Aplikativna in bazična fiziologija in patofiziologija v medicini