Magnetic resonance imaging for rapid screening for the nephrotoxic and hepatotoxic effects of microcystins

Milutinović Živin, Aleksandra (author), Zorc-Pleskovič, Ruda (author), Živin, Marko (author), Vovk, Andrej (author), Serša, Igor (author), Šuput, Dušan (author)

In vivo visualization of kidney and liver damage by Magnetic Resonance Imaging (MRI) may offer an advantage when there is a need for a simple, non-invasive and rapid method for screening of the effects of potential nephrotoxic and hepatotoxic substances in chronic experiments. Here, we used MRI for monitoring chronic intoxication with microcystins (MCs) in rat. Male adult Wistar rats were treated every other day for eight months, either with MC-LR (10 μg/kg i.p.) or MC-YR (10 μg/kg i.p.). Control groups were treated with vehicle solutions. T$_1$-weighted MR-images were acquired before and at the end of the eight months experimental period. Kidney injury induced by the MCs presented with the increased intensity of T$_1$-weighted MR-signal of the kidneys and liver as compared to these organs from the control animals treated for eight months, either with the vehicle solution or with saline. The intensification of the T$_1$-weighted MR-signal correlated with the increased volume density of heavily injured tubuli (R$^2$ = 0.77), with heavily damaged glomeruli (R$^2$ = 0.84) and with volume density of connective tissue (R$^2$ = 0.72). The changes in the MR signal intensity probably reflect the presence of an abundant proteinaceous material within the dilated nephrons and proliferation of the connective tissue. T$_1$-weighted MRI-is a valuable method for the in vivo screening of kidney and liver damage in rat models of intoxication with hepatotoxic and nephrotoxic agents, such as microcystins.

• Language: English
• Keywords: microcystin, chronic toxicity, magnetic resonance imaging, kidney, liver, nephrotoxic
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2013
• Number of pages: Str. 2785-2798
• Numbering: Vol. 11, iss. 8
• PID: 20.500.12556/RUL-129250
• UDC: 577.1
• ISSN on article: 1660-3397
• DOI: 10.3390/md11082785
• COBISS.SI-ID: 30744025
• Publication date in RUL: 31.08.2021

Record is a part of a journal

• Title: Marine drugs
• Shortened title: Mar. drugs
• Publisher: MDPI
• ISSN: 1660-3397
• COBISS.SI-ID: 23578841

Licences

• License: CC BY 3.0, Creative Commons Attribution 3.0 Unported
• Link: https://creativecommons.org/licenses/by/3.0/deed.en
• Description: You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
• Licensing start date: 05.08.2013

Secondary language

• Language: Slovenian
• Keywords: mikrocistin, kronična toksičnost, slikanje z magnetno resonanco

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0019
• Name: Aplikativna in bazična fiziologija in patofiziologija v medicini