Cannabis is the most widespread and abused drug in the World and has been
known to humanity for thousands of years. Its most known constituents, cannabinoids,
play a direct role in interacting with the human endocannabinoid system. Relatively
recently however, their synthetic counterparts have spread across the globe and are
more difficult to detect with the usual analytical methods. One of the better instruments
for determination of cannabinoids is the HPLC-QTOF. In the thesis we used the HPLC-QTOF and optimized a method for analysis of natural and synthetic cannabinoids. We
partially validated the method and demonstrated that it can also be used for quantitative
analysis in different matrixes. We have shown fragmentation pathways of cannabinoids
and proposed some mechanisms. We also performed an analysis of real samples and
their comparison.
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