Analiza sintetičnih in naravnih kanabinoidov v vzorcih prepovedanih drog in izdelkih iz konoplje

Kragolnik, Goran (author), Kolar, Mitja (mentor)

Konoplja je najbolj razširjena ter uporabljena droga na svetu in je človeštvu znana že več tisoč let. Kanabinoidi, ki so njene najbolj znane sestavine, igrajo neposredno vlogo pri interakciji z endokanabinoidnim sistemom pri ljudeh. V relativno kratkem času so se po svetu razširile tudi njihove sintetične različice, ki jih je težje identificirati z običajnimi analiznimi metodami. Eden boljših sistemov za določanje kanabinoidov je HPLC-QTOF. V diplomskem delu smo z HPLC-QTOF optimizirali metodo za analizo naravnih in sintetičnih kanabinoidov. Metodo smo delno validirali in dokazali, da jo je možno uporabiti tudi za kvantitativno analizo v različnih matricah. Prikazali smo fragmentacijske poti kanabinoidov in predlagali nekaj mehanizmov. Izvedli smo tudi analize realnih vzorcev in medsebojno primerjavo le-teh.

• Language: Slovenian
• Keywords: konoplja, naravni in sintetični kanabinoidi, HPLC-QTOF, validacija metode, fragmentacijski mehanizmi
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2019
• PID: 20.500.12556/RUL-109663
• COBISS.SI-ID: 1538328259
• Publication date in RUL: 06.09.2019

KRAGOLNIK, Goran, 2019, Analiza sintetičnih in naravnih kanabinoidov v vzorcih prepovedanih drog in izdelkih iz konoplje [online]. Bachelor’s thesis. [Accessed 11 April 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=109663

Secondary language

• Language: English

Abstract

Cannabis is the most widespread and abused drug in the World and has been known to humanity for thousands of years. Its most known constituents, cannabinoids, play a direct role in interacting with the human endocannabinoid system. Relatively recently however, their synthetic counterparts have spread across the globe and are more difficult to detect with the usual analytical methods. One of the better instruments for determination of cannabinoids is the HPLC-QTOF. In the thesis we used the HPLC-QTOF and optimized a method for analysis of natural and synthetic cannabinoids. We partially validated the method and demonstrated that it can also be used for quantitative analysis in different matrixes. We have shown fragmentation pathways of cannabinoids and proposed some mechanisms. We also performed an analysis of real samples and their comparison.

• Keywords: cannabis, natural and synthetic cannabinoids, HPLC-QTOF, method validation, fragmentation mechanisms