Vpliv bisfenolov A in F na rast alg in cianobakterij

Notersberg, Tilen (avtor), Marinšek Logar, Romana (mentor), Eleršek, Tina (komentor)

Namen te magistrske naloge je bil ugotoviti vpliv bisfenola A in F na stopnjo rasti evkariontske mikroalge Pseudokirchneriella subcapitata in prokariontske cianobakterije Synechococcus leopoliensis. Mikroalge in cianobakterije smo za tri dni izpostavili različnim koncentracijam bisfenola A in F. Vsak dan smo izmerili gostoto celic s pretočnim citometrom, na koncu poskusa pa smo še s plinsko kromatografijo sklopljeno z masno spektrometričnim detektorjem preverili, če se je tekom poskusa v gojišču spremenila koncentracija testiranih bisfenolov. Ugotovili smo, da je zaviranje rasti odvisno od koncentracije bisfenolov. Za algo P. subcapitata smo izmerili naslednje ekotoksikološke podatke: EC50 = 6,8 mg/l, NOEC = 4,9 mg/l, LOEC = 15,6 mg/l za BPA in EC50 = 9,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l za BPF. Za cianobakterijo S. leopoliensis pa smo izmerili EC50 = 4,8 mg/l, NOEC = 0,5 mg/l, LOEC = 1,5 mg/l za BPA in EC50 = 5,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l za BPF. Rezultati kažejo, da ima BPF podobne učinke na alge in cianobakterije kot BPA in zato z okoljskega stališča ni primerna zamenjava za BPA.

• Jezik: Slovenski jezik
• Ključne besede: alge, mikroalge, cianobakterije, Pseudokirchneriella subcapitata, Synechococcus leopoliensis, bisfenol A, bisfenol F, rast
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Založnik: [T. Notersberg]
• Leto izida: 2019
• PID: 20.500.12556/RUL-107949
• UDK: 502/504:582.26:579.24
• COBISS.SI-ID: 5059448
• Datum objave v RUL: 10.06.2019

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: The infulence of bisphenol A and F on algal and cyanobacterial growth

Izvleček

The purpose of this masters thesis was to find out the effect of bisphenol A and F on the growth rate of eukaryotic microalgae Pseudokirchneriella subcapitata and prokaryotic cyanobacteria Synechococcus leopoliensis. Microalgae and cyanobacteria were exposed to different concentrations of bisphenol A and F for three days. During incubation, every day cell density was measured with a flow cytometer and, at the end of the experiment, bisphenol concentration was determined by gas chromatography coupled with a mass spectrometric detector to determine if their concentration changed during the experiment. We found that growth inhibition is concentration dependent. We obtained the following ecotoxicological values for P. subcapitata EC50 = 6,8 mg/l, NOEC = 4,9 mg/l, LOEC = 15,6 mg/l for BPA and EC50 = 9,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l for BPF. For S. lepopoliensis the values were EC50 = 4,8 mg/l, NOEC = 0,5 mg/l, LOEC = 1,5 mg/l for BPA and EC50 = 5,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l for BPF. Our result show that BPF has similar effects on algae and cyanobacteria as BPA and is therefore not a suitable replacement for BPA from an environmental point of view.

• Ključne besede: algae, microalgae, cyanobacteria, Pseudokirchneriella subcapitata, Synechococcus leopoliensis, bisphenol A, bisphenol F, growth