The purpose of this masters thesis was to find out the effect of bisphenol A and F on the growth rate of eukaryotic microalgae Pseudokirchneriella subcapitata and prokaryotic cyanobacteria Synechococcus leopoliensis. Microalgae and cyanobacteria were exposed to different concentrations of bisphenol A and F for three days. During incubation, every day cell density was measured with a flow cytometer and, at the end of the experiment, bisphenol concentration was determined by gas chromatography coupled with a mass spectrometric detector to determine if their concentration changed during the experiment. We found that growth inhibition is concentration dependent. We obtained the following ecotoxicological values for P. subcapitata EC50 = 6,8 mg/l, NOEC = 4,9 mg/l, LOEC = 15,6 mg/l for BPA and EC50 = 9,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l for BPF. For S. lepopoliensis the values were EC50 = 4,8 mg/l, NOEC = 0,5 mg/l, LOEC = 1,5 mg/l for BPA and EC50 = 5,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l for BPF. Our result show that BPF has similar effects on algae and cyanobacteria as BPA and is therefore not a suitable replacement for BPA from an environmental point of view.
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