Določevanje beta-glukanov v vzorcih ječmena in pšenice

Kuzman, Rok (author), Cigić, Blaž (mentor), Polak, Tomaž (reviewer)

Namen diplomske naloge je določiti vsebnost β-glukanov v zrnih različnih kultivarjev pšenice in ječmena in s tem identificirati kultivarje z veliko vsebnostjo β-glukanov, ki jih bo možno uporabiti za pripravo specialnih mok. β-glukani so sestavni del celične stene različnih žit, predvsem v celicah, ki tvorijo sub-alevronsko plast. Selekcija žit, ki je temeljila na velikem hektarskem donosu in veliki vsebnosti škroba in proteinov, je rezultirala v naboru kultivarjev, ki imajo relativno majhno vsebnost β-glukanov. V zadnjem času pa je mnogo raziskav pokazalo na pozitivne učinke β-glukanov na zdravje. Posledično je Evropska agencija za varnost hrane odobrila tudi dve prehranski trditvi v povezavi z β-glukani. Zaradi naštetega obstaja velik interes za gojenje žit kot so pšenica in ječmen z veliko vsebnostjo β-glukanov. Z analizami ječmena smo ugotovili, da zrna izbranih kultivarjev vsebujejo od 1,6 do 9,0 % β-glukanov, medtem ko je bila vsebnost v zrnih pšenice od 0,56 % do 0,97 %.

• Language: Slovenian
• Keywords: ječmen, ječmenova moka, beta-glukani iz žit, LDL holesterol, izolacija beta-glukanov, funkcionalna živila
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [R. Kuzman]
• Year: 2017
• PID: 20.500.12556/RUL-95629
• UDC: 633.1:664.641:547.458:641.1
• COBISS.SI-ID: 4835704
• Publication date in RUL: 21.09.2017

Secondary language

• Language: English
• Title: Analysis of beta-glucan content in barley and wheat samples

Abstract

The aim of the thesis is to identify the content of β-glucans in the grains of different samples of wheat and barley and thus identify cultivars which can be used to produce special flour. β-glucans are found in sub-aleuronic layer of grain's cellular wall. So far, a lot of effort has been put into making greater yield per hectare as well as higher content of starch and proteins and thus the content of β-glucans in grains decreased. Lately many researches have shown positive effects of β-glucans on human health. Consequently European Food Safety Authority support two health claims regarding positive effects of β-glucans on human health. This information has increased the interest for growing cultivars with higher contents of β-glucans. We have found than the content of β-glucans in analysed barley cultivars is from 1.6 % to 9.0 % and in wheat cultivars from 0.56 % to 0.97 %.

• Keywords: barley, barley flour, beta-glucans from grain, LDL cholesterol, isolation of beta-glucans, functional food