Protocols for micropropagation of iboga Tabernanthe iboga (L.) Nutt. was studied by various methods of plant tissue culture. One them was hormone-free, the other contained auxins IBA or NAA. Seeds germinates on all germination media and seedlings developed into plants. Multiplication of shoots was tested on various compositions of macro and micro elements - B5, WPM, MS, DKW. Medium RP1 (B5 medium) was the best for shoots multiplication. On rooting media no rooting occurred but formation of callus. Leaf yellowing was later reduced in media containing iron chelated with FeEDDH. Unexpectedly in liquid media supplemented with auxins somatic formation of embryos occurred. Induction of root growth from shoot and root explants was tested on solid and liquid media. On solid media growth was slow but root explants formed a limited quantity of new roots and shoots. Roots cultured in liquid media formed additional roots but after third subculture somatic embryos were formed. Somatic embryos were formed in several subcultures and were grown to plantlet stage being successfully acclimatized. The results of measurements of ibogaine was received from Dr. Gordane Koželj from the Institute of Forensic Medicine Faculty of Medicine, University of Ljubljana, the "green pattern" (shoots) containing 0.010 wt. % Ibogaine, "brown sample" (the roots of the plant) 0.012 wt. % ibogaine.
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