We have studied the influence of UV radiation and selenium (Se) on selected morphological, biochemical and optical properties of leaves in hybrid buckwheat. These hybrid buckwheat were exposed to four different treatments, with UV filters and different solutions of natrium selenat. Firstly, we have used ambient UV radiation without added selenium; secondly, we used ambient UV radiation with foliarly sprayed selenium; thirdly, we used reduced UV radiation without selenium, and lastly we used reduced ambient UV radiation with added selenium. We have measured photochemical efficiencies of PS II and ETS activity, reflectance and trasmittance, and determined clorophyll a and b, carotenoids and anthocyanins in invastigated leaves. We observed the influence on dry and fresh mass, in underground and overground parts of plant, and also determined morphological and anatomical leaf properties. The treatments influenced the transpiration, potential photochemical efficiency of PS II and activity of terminal electron transport system. The treatmentsexcited less effect on morphological poperties, because it was observed only in fresh leaves. Influence of interacions of both factor is shown in stoma and trychomes. The way we treated the plants had influenced concentrations of pigmentation, because plants that were treated with Se+UV+the lowest concetration of chlorophyll a, clorophyll b and carotenoids. Concentration of antocians in UV-A and UV-B absorbing substances was the lowest in plants that were treated with Se-UV-. Treatmets had also had impact on optical properties, because plants treated with Se+UV+ had the highest reflectance and transmittance in every aspect.
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