Biological cell is a main unit of all living organisms. It consists of cell nucleus, cytoplasm and cell membrane that envelope the cell and protect it from the surrounding. Electroporation is a method where cells exposed to electric field becomes more permeabilized. Therefore, lager molecules, drugs and DNA enables to enter the cell that in normal condition would not penetrate.
Planar lipid bilayer can be studied as a simple model of a cell membrane. It is stipulated that electroporation provokes changes on a lipid bilayer part of membrane. Elucidated understanding of electroporation of planar lipid bilayers helps to explain occurrence on the cell membrane under electric field. Planar lipid bilayer can be considered as a good model of a cell membrane and it is electrically accessed from the both sides. For measuring planar lipid bilayer properties, the voltage or current clamp sources and transmembrane voltmeter and ampere meter is required.
The main work of this thesis was to construct and design a printed board circuit of integrated measurement system device for measuring properties of planar lipid bilayer. The device is consisted of open source credit card sized measurement computer Red Pitaya, amplifiers of voltage and current clamp, amplifiers for measurement transmembrane voltage and current, peripheral circuit for pump control, temperature control, power supply and TTL output for triggering an external nanosecond pulse generator.
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