Razvoj metode za HPLC-analizo izbranih fenolnih spojin lesa

Poljanšek, Ida (author), Vek, Viljem (author), Oven, Primož (author)

Preučevali smo vpliv sestave mobilne faze na ločitev enostavnih fenolnih spojin, kot so galna kislina, pirokatehol, floroglucinol, resorcinol, vanilin in flavonoida katehina s tekočinsko kromatografijo visoke ločljivosti (HPLC). Kot najbolj primerna metoda za ločitev komponent se je izkazala metoda gradientnega izpiranja s programiranim spreminjanjem sestave mobilne faze acetonitril in 2-odstotna vodna raztopina mravljinčne kisline od 2 : 98 do 25 : 75. Ločitev organskih spojin je bila učinkovitejša v mobilni fazi z manjšim deležem mravljinčne kisline. HPLC je primerna metoda za identifikacijo in kvantitativno analizo spojin v različnih tipih vzorcev na širokem področju verige gozdarstva, lesarstva in papirništva.

• Language: Slovenian
• Keywords: les, ekstraktivi, fenolne spojine, katehin, kromatografija, HPLC, ločba
• Work type: Scientific work
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2015
• Number of pages: Str. 11-17
• Numbering: 108
• PID: 20.500.12556/RUL-81620
• UDC: 630*84:547.562.4
• ISSN on article: 2335-3112
• DOI: 10.20315/ASetL.108.2
• COBISS.SI-ID: 4357030
• Publication date in RUL: 29.04.2016

Record is a part of a journal

• Title: Acta Silvae et Ligni
• Publisher: Gozdarski inštitut Slovenije, založba Silva Slovenica, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
• ISSN: 2335-3112
• COBISS.SI-ID: 266761216

Secondary language

• Language: English
• Title: Development of the HPLC method for separation of some phenolic compounds of wood

Abstract

The most suitable composition of mobile phase for the separation of simple phenolic compounds such as gallic acid, pyrocatechol, phloroglucinol, resorcinol, vanillin and related compound catechin by the high performance liquid chromatography was investigated. The most appropriate developing mobile system for the separation of these organic compounds was acetonitrile and 2% water solution of formic acid with gradient ratio from 2.98 to 25:75. The best separation of the six organic compounds was achieved using mobile phase with lower content of formic acid. HPLC is a method of choice for qualitative and quantitative analysis compounds in various types of samples in the broad field of forestry-wood-pulp-paper chain.